| ObjectiveGastric cancer is one of the most common malignancies of gastrointestinal tract. Theroles of chemokines and their receptors in gastric cancer have attracted more and moreinterests.Chemokines belong to a kind of cytokine family of small molecular secreted protein.The function of chemokines is performed as chemotaxis. Following the first identifiedchemokine, more than50chemokines have been found. Three-dimensional structures ofmost chemokines are similar. The formation of its amino acid sequence contains4conserved cysteines and two characteristic disulfide bond. According to the differentspatial structure, chemokines can be divided into4categories: C, CC, CXC and CX3C.Chemokine receptors are GTP-protein-coupled transmembrane receptors (GPCR), whichcan mediate the function of chemokines. Chemokine receptors are usually expressed on themembrane of immune cells and endothelial cells. Chemokine receptors can be dividedinto the following categories: type1. CC receptor (CCR): CC chemokines binding receptor;2.CXC receptor (CXCR): binding with the CXC chemokines;3.C and CX3C receptors(CR, CX3CR). The combination of chemokines and their receptors not only can inducetarget cells directional migration and rearrangement of the cytoskeleton, but also enhancethe adhesion ability of target cells and endothelial cells. Chemokines and their receptorsexert various physiological functions on involved cells.In this study, expression of CCL20, CCR6, CXCL10and CXCR3in gastric cancerwas detected and the relationship with the clinical characteristics of gastric cancer was alsoanalyzed, in order to investigate the relationship between chemokine/receptor and thedevelopment of gastric cancer, laying the foundation of the future treatment and prognosisof gastric cancer. MethodsTissue specimens from50patients with gastric cancer and20healthy controls werecollected from Jan.2011to Jan.2012at Qingdao Municipal Hospital. Expressions ofCCL20/CCR6, CXCL10/CXCR3were determined by immuno-histochemistry, and theirrelationships with clinical pathological features of gastric cancer were also analyzed.ResultsExpression of CCL20in gastric cancer tissues, paracancerous tissues was higher thanthat in normal gastric mucosa, and the positive rates were56%(28/50),16%(8/50) and10%(2/20), respectively. Positive rates of expression of CCR6in gastric cancer tissues,paracancerous tissues and normal gastric mucosa were64%(32/50),14%(7/50) and15%(3/20) respectively. There were significant differences between expression of CCR6ingastric cancer tissues and that in normal gastric mucosa. The positive rates of expression ofCXCL10in gastric cancer tissues and paracancerous tissues were60%(30/50) and14%(7/50).There was statistical significance between these two groups. The positive expressionof CXCL10in normal gastric mucosa was10%(2/20). There was significant differencebetween the expression of CXCL10in gastric cancer tissues and that in normal gastricmucosa. The positive rates of expression of CXCR3in gastric cancer tissues andparacancerous tissues were58%(29/50) and18%(9/50).There was statistical significancebetween these two groups. The positive expression of CXCR3in normal gastric mucosawas10%(2/20).There was significant difference between the expression of CXCR3ingastric cancer tissues and that in normal gastric mucosa.Expression of CCL20and CXCR3had significant correlation with depth of invasion,but had no correlation with age, gender, differentiation degree and tumor stage. Expressionof CCR6and CXCL10had significant correlation with invasion depth, but had nocorrelation with age, gender, degree of differentiation.ConclusionCCL20/CCR6and CXCL10/CXCR3are over-expressed in gastric cancer tissues, andhad associated with depth of invasion. These showed that CCL20/CCR6andCXCL10/CXCR3are involved in the development of gastric cancer, contributing to thefuture prognosis and the treatment options. |
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