| Background: Gastric cancer as the most common malignant tumors in the humandigestive system, is characterized by insidious onset, early metastasis, high mortality,serious threat to the lives and health of patients. Radical gastrectomy develop andmature, and rational application of the chemotherapy drugs, the five-year survival rateof patients with gastric cancer has increased, But our patients with advanced gastriccancer metastasis and recurrence rate is still stubbornly high. Peritoneal metastasis isone of the important reasons leading to the death of patients with advanced gastriccancer, also important and difficult in the treatment of gastric cancer. Peritonealmetastasis is the result of the role of physicochemical factors. Studies have shown thatchemokines, as a small molecule peptide can chemotactic cell movement, played animportant role in various tumors growth and metastasis. CXCL12/CXCR4biologicalaxis is associated with a variety of tumors, CXCR4has been considered to be the solereceptor of CXCL12, however, recent studies have found that CXCL12having anothernew receptor CXCR7. This finding not only subvert the point view of CXCL12andCXCR4-specific binding, but also CXCR7be deemed to play an important role intumor development. At the same time, a large number of studies have shown thatanother chemokine receptor CCR5, also play an important role in tumor growth andmetastasis of breast cancer, colorectal cancer and many other cancer.Objective: By immunohistochemical methods to detect chemokine receptorCXCR7and CCR5,in paracarcinomatous tissue, primary lesion, peritoneal metastaticlesion.Comparison the expression of CXCR7and CCR5in primary lesion,gastriccancer patients with peritoneal metastasis and non peritoneal metastasis, Explore itsrelationship with peritoneal metastasis. Methods: Selected43cases of peritoneal metastasis of gastric cancer patients,surgery in the period from January2002to September2011in Dalian MedicalUniversity, required to have primary tumor, peritoneal metastasis, to haveparacarcinomatous tissue (more than5cm from the tumor lesions). Another50caseswere randomly selected over the same period without peritoneal metastasis of gastriccancer specimens. All of the cases were not conducted in the chemotherapy beforesurgery. Specimens were fixed in formalin, embedded in paraffin afterimmunohistochemical method for the determination of chemokine receptors CXCR7,CCR5expression. Univariate analysis the expression of CXCR7and CCR5inperitoneal metastatic lesions to explore the relationship with clinicopathologicalparameters. Using a logistic regression model to analyze the risk of variousclinicopathological parameters of peritoneal metastasis.Results: Chemokine receptor CXCR7, CCR5is expressed mainly in the cytoplasmand membrane of tumor cells. In43patients with peritoneal metastasis cases, theexpression rates of CXCR7in paracarcinomatous tissue,primary tumor, peritonealmetastatic lesion were:9.3%，74.4%，93%，expression of the three organizations havesignificant differences (p <0.05), Have Statistically significant; the expression rates ofCCR5in paracarcinomatous tissue, primary tumor,and peritoneal metastatic lesion were:27.9%，81.4%，88.4%, CCR5in peritoneal metastatic lesion and primary tumors, theexpression was not significantly different (p>0.05). CCR5in primary tumors andadjacent tissues are significantly different (p <0.05). In50gastric cancer patientswithout peritoneal metastasis in primary tumor specimens of CXCR7, CCR5expression rates were:32%,60%. Without peritoneal metastasis and peritonealmetastasis of primary tumor specimens between the CXCR7, CCR5expression wassignificantly different (p<0.05).Univariate analysis of CXCR7and CCR5in the relationship between theexpression and the clinicopathological parameters of gastric cancer peritonealmetastases: CXCR7in gastric cancer peritoneal metastasis were correlated with tumorsize, differentiation, tumor invasion depth-related (p <0.05), with the patient’s age, sex,tumor location, lymph node metastasis had no correlation (p>0.05); CCR5in gastriccancer peritoneal metastasis were correlated with lymph node metastasis,differentiation, tumor invasion depth-related (p <0.05), with the patient’s age, sex,tumor location, tumor size had no correlation (p>0.05); Multi-factor regression analysisshowed that: the primary tumor size, depth of invasion, differentiation, CXCR7expression in primary tumors is a risk factor for peritoneal metastasis of gastric cancer. Peritoneal metastasis and no peritoneal metastasis of gastric cancer compared in thetumor size, depth of invasion, lymph node metastasis differentiation were significantlydifferent (p <0.05); and in age,gender, tumor site areas no difference (p>0.05).Conclusion: Chemokine receptor CXCR7is expressed in primary lesion andmetastatic lesion,only a small amount of expression in normal gastric mucosa,theexpression of CXCR7in the three tissues are significant differences，which peritonealmetastatic lesion positive expression rate of the highest.CXCR7in gastric cancerperitoneal metastasis were correlated with tumor size, differentiation, tumor invasiondepth-related, with the patient’s age, sex, tumor location, lymph node metastasis had nocorrelation; Chemokine receptor CCR5is expressed in primary lesion and metastaticlesion, few less CCR5expression in normal gastric mucosal，the expression rates ofprimary lesion and metastatic lesion focus are significantly higher than the mucosaltissue paraneoplastic,There are significant differences, but no significant difference inexpression between the primary lesion and metastatic lesion.CCR5in gastric cancerperitoneal metastasis were correlated with lymph node metastasis,differentiation, tumorinvasion depth-related, with the patient’s age, sex, tumor location, tumor size had nocorrelation.These tips chemokine receptor CXCR7and CCR5may promote gastriccancer progression and metastasis，In particular, CXCR7, as one of the risk factors forperitoneal metastasis may in the peritoneal metastasis play an important role. |
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