Xiongbitong Capsule On Myocardial Ischemia Reperfusion Injury In Rats CaM

Objective：The study of xiong bi tong capsule on myocardial ischemia reperfusionmyocardial CaM,and to explore its possible mechanism of myocardial ischemiareperfusion injury to provide a theoretical basis for clinical medical.Methods: The rats were randomly divided into three groups: sham operation group,model group and xiongbitong capsule group (n=10). Constructed by ligation of the leftanterior descending coronary artery myocardial ischemia-reperfusion injury model (1) theuse of the ELISE assay experimental rat cardiac CaM content changes;.(2) use of theautomatic analyzer experimental serum creatine kinase CK, creatine kinase isoenzymeCK-MB content changes;(3) Utilize spectrophotometer experimental rat myocardiummalondialdehyde (MDA) content, superoxide dismutase (SOD) activity changes;(4) bymeans of optical microscopy and electron microscopy of myocardial ischemia-reperfusioninjury in rat myocardial cell morphology and ultrastructure change.Results:(1) xiongbitong capsule enables CaM significantly discreased, with the I/Rgroup control, the difference was statistically significant (P <0.05);(2) xiongbitongcapsule can effectively inhibit myocardial cell damage caused by reperfusion reduce CKand CK-MB leakage control and I/R group, the difference was statistically significant (P<0.05, P <0.05);(3) the xiongbitong capsule can significantly inhibit the production ofMDA, and SOD activity can be increased, with the I/R group control, the difference wasstatistically significant (P <0.05, P <0.05);(4) observation of cell morphology andultrastructure, xiongbitong capsule can effectively protect the myocardial tissue structure,inhibition of reperfusion injuryConclusions:(1) xiongbitong capsule can inhibit the production of MDA, andsignificantly increased activity of SOD can reduce the damage of oxygen free radicals,effective protection of myocardial tissue;(2) The xiongbitong capsule can effectively improve the myocardial tissue reperfusion injury caused structural damage, reduce CK andCK-MB leakage of protecting myocardial tissue;(3) xiongbitong capsule enables themyocardium of CaM significantly discreased the protection of the myocardial tissue.