The Relationship Between Periodontitis And Content Of IL-6Level And PON1Activity In Serum

Objective: Through the observation and analysis of the effect of basicperiodontal therapy on serum interleutin-6(IL-6) level and serum paraoxonase-1(PON1) activity in advanced periodontitis to explore the possible relationship ofperiodontitis and content of IL-6level and PON1activity in serum, even theirpossible effect in the change of periodontitis, in this way a theoretical basis isprovided for the further study on periodontitis impact on general health.Methods:1.object of study85people including periodontally healthy volunteers and patients who werediagnosed advanced periodontitis in Liaoning Provincial People,s Hospital fromMay2012to February2013were enrolled in the study. Accordance with the strictinclusion criteria and exclusion criteria can be divided into two group: periodontalhealthy group and advanced periodontitis group. To eliminate the16people whodidn,t accept return visit after three months of basic periodontal therapy, Resultstotally69subjects were recruited and were divided into two groups: periodontalhealthy group (group A, n=30) and advanced periodontitis group (group B,n=39).Both group received basic periodontal therapy, named group A as group A1and group B as group B1after3months.2.general checking-up and data collectionProcess includes questionnaires, periodontal examination, the collection anddetection of serum samples.(1) Questionnaires: age, gender, weight,height,drinking and so on.(2) Periodontal examination: By the same dentist using the periodontology(fourth edition), published by the people,s medical as a standard to exam periodontalcondition of six index teeth from four range inside the mouth and recorded theirperiodontal clinical parameters(BI,PD,AL). (3) Imaging examination: buccal panoramic radiography.(4) Collecting and detecting serum: after serum collected completely to examineIL-6level by the method of enzyme-linked immunosorbent assay (ELISA) reactionand detect serum PON1activity with automatic analyzer method.3. intervene treatmentPeriodontal healthy group: oral health instruction, remove supragingival calculusand plaque bacteria from the surface, local3%H2O2flushing and besmear iodicglycerine.Advanced periodontitis group: oral health instruction, supragingival scaling,subgingival scaling and root planning, local3%H2O2flushing and besmear iodicglycerine.4. data statisticsUse SPSS13.0software to make a statistical analysis of the data collected. Thet-test was applied in the intergroup comparison. Correlation analysis usingchi-square test. P<0.05was considered to be statistically significant．Results:1. The contrast analysis of results before the basic periodontal therapy:Comparing group A and group B, when the patients age, gender, ration of drinkingin each group, body mass index were in a similar and general level. Serum IL-6levelof group B was much higher than that in group A. The difference was statisticallysignificant(P<0.01). Serum PON1activity in two groups didn,t appeared asignificant differences(P<0.05).2. The contrast analysis of results before and3months after basic periodontaltherapy: Comparing group A and group A1, serum PON1activity and IL-6level wasno significant differences. Periodontal clinical parameters (BI,PD)and serum IL-6level had a significant fall in the group B1compared with group B（P<0.05).The ALhad a mild improvement in the group B1compared with group B, but didn,tappeared a significant differences. Serum IL-6level had a significant fall in thegroup B1compared with group B. PON1activity in Serum of group B1was muchhigher than that in group B. The difference was statistically significant(P<0.05).3. The correlation analysis of each examination index before and after the basicperiodontal therapy: The IL-6level in serum was positively associated withperiodontal clinical parameters (BI、�'＝0.68,P<0.05； PD、�'＝0.71,P<0.05）. The IL-6level changes in serum was negatively associated with the PON1activitychanges in serum before and after the basic periodontal therapy.Conclusion:1. Serum PON1activity in periodontal healthy group and advanced periodontitisgroup didn,t appeared a significant differences, but serum PON1activity could beincreased through the periodontal foundation treatment in patients with advancedperiodontitis. It indicates that relatively large differences in the expression of PON1in different individuals.2. The periodontal foundation treatment is helpful in improving periodontalstatus and increasing the serum PON1activity while decreasing the serum IL-6levelin patients with advanced periodontitis.It indicates that PON1activity and IL-6levelmay effect the periodontitis diseases.3. The IL-6level changes in serum was negatively associated with the PON1activity changes in serum before and after the basic periodontal therapy.It indicatesthat the higher PON1activity may possesses the anti-inflammation function in theperiodontitis.