The Empirical Study Of The Effects Of Different Time Of Exercise On Neurological Function Recovery After Intracerebrai Hemorrhage In Rats

Objective:To investigate the optimal time of movement after experimental intracerebral hemorrhage in rats,and to provide directions for cerebral hemorrhage patients in clinic.Revealing the possible mechanism of why exercise training can promote rats’ neural function and exercise capacity after cerebral hemorrhage.Methods:Male SD rats were randomly divided into control group,model group and experiment group.The experimental group rats were divided into three group,including12hours of exercise group,4days of exercise group and8days of exercise group according to the time of starting exercise.Model group and experimental group rats were made into model of cerebral hemorrhage by injecting the collagenase and heparin into the caudate nucleus of rats,brain.In the control group,collagenase and heparin were instead by normal saline,and rats in the model group and the control group did not have exercise training.The rats in each group were divided into3days,7days,14days and21days subgroup according to the time of being killed.The rats in each group were istimated by NSS scores sistem immediately after the recovery from anesthesia.Only the rats attaining certain scores can be divided into the experiment. The HE staining method was used to observe the morphological changes of the tissue around the hematoma of rats.The expression of NGF and(?) Caspase3around the hematoma of rats were measureed by immunohistochemical method.Results:(1) The NSS score of the12hours of exercise group is higher than the model group in the3、7、14、21day,the difference was statistically significant(P<0.05); The NSS score of the4day of exercise group is less than the model group in the14、21day,the difference was statistically significant(P<0.05); The NSS score of the8day of exercise group is less than the model group in the14day,the difference was statistically significant(P<0.05).(2) There are different degrees of shrinkage, condensed, degeneration and necrosis in The nerve nuclei of model group,and there are varying of intercellular vacuoles,edema and inflammatory cell infiltration,surrounding the tissue,and glial cells and vascular hyperplasia was major.The12hours of exercise group of brain edema and nerve cell necrosis and inflammatory cell infiltration intensity was higher than the model group.The degree of4days group and8days group of nerve cell necrosis, inflammatory cell infiltration intensity are lower than the model group at each time,but glial cells and vascular proliferation was higher than the model group.(3) Compare with control group, the expression of NGF of model group were increased significantly on the3、7、14days,The difference was statistically significant (P<0.05).;the expression of NGF of12hours of group,4days group and8days group NGF were higher than the control group in the14、21days and the difference was statistically significant (P<0.05).(4) The expression of Caspase-3of model group was higher than the control group at the3、7、14days, and the difference was statistically significant(P<0.05). The expression of Caspase-3of the12hours group was higher than the model group at the14days, and the difference was statistically significant(P<0.05); The expression of Caspase-3of the4days group was less than the model group at the7、14days, and the difference was statistically significant(P<0.05); The expression of Caspase-3of the8days group was much less than the model group at the14days, and the difference was statistically significant(P<0.05). Conclusions:(1) After intracerebral hemorrhage in rats, premature exercise training intervention increased rats, damage level of nerve function.(2)The updated sports training plays a positive role in nerve function after suffering cerebral hemorrhage.(3) the movements,role of increasing the expression of NGF and Inhibiting the expression of Caspase-3may be one of the mechanisms to promote the recovery of cerebral hemorrhage.