Influence Of Rosuvastatin On Caspase-9and C-IAP-1of Experimental Intracerebral Hemorrhage In Rats

OBJECTIVE:To observe the neuroprotective effect of rosuvastain and its influence to Caspase-9and tissue inhibitors of C-IAP-1in intracerebral hemorrhage (ICH) rat, and to explore the possible mechanism of its neuroprotective effect.METHODES:Fifty Spragne Dawley(SD) rats were randomly selected and divied into2groups:ICH group(A group), ICH atrovastain therapy(B group). To utilize stereotaxic apparatus and infuse fresh quantized ICH model, Treatment groups were atrovastin(10mg/kg/d) for seven days. As comparing research, ICH groups were administered with phosphate-buffered saline for seven days.The expression of caspase-9, C-IAP-1in Rats’brain at various time points after operation were analyzed by enzyme linked immuno-histochemistry (IP) and the neurological behaviors of rats were observed by measurement of longa scores. The expressions of Caspase-9, C-IAP-1in brain tissue were determined with IP. The pathological change of brain tissue were observed under light-microscopy.RESULTS:1.。l.At the same time point, neurological deficits of two groups reduced significantly(P<0.05),and A group performed significant difference with group B post ICH (P<0.05).2. In comparison with A group, the number of activated microglia of group B were significantly reduced(P<0.05). Rosuvastains caused a dose-dependent regulation, which decreased the level of Caspase-9and increased the level of C-IAP-1significantly (P<0.05).CONCLUSIONS:1. Our study suggested that rosuvastain may reduces secondary neurological deficits of cerebral hemorrhage, and promotes the recovery of neurological function.2. Rosuvastatin can decrease the level of the Caspase-9and increase the level of C-IAP-1.It can also inhibit cellular stress reaction in early stage of cerebral hemorrhage, and reduce the secondary brain damage caused by apoptotic response.All of these maybe its mechanism of neuroprotective effects.