The Expression And The Preliminary Functional Studies Of MiR-145in Renal Carcinoma And Bladder Tumor

Objective:To explore the expression of miR-145in the renal cell carcinomas and renal cancer cell lines, bladder tumors and its cancer cell lines. Moreover, we want to expound the roles of miR-145in tumor metastasis and identify a novel mechanism of miR-145to suppress the invasion-metastasis cascade in renal tumor and bladder cancer.Method:Using RT-qPCR technology to explore the expression levels of miR-145in the tissues of renal carcinomas and their nontumorous tissues, in the renal cancer cell lines such as786-0、ACHN、SN12-PM6、Caki-1. Later, using the same technology to investigate the quantitation of miR-145expression levels in the tissues of bladder cancer and their nontumorous tissues, also including the cell lines for example5637、T24、 HT1376. The migration assay was performed with Transwell inserts that have6.5mm polycarbonate membranes with pores8.0μm in size in order to find the roles of miR-145in the tumor metastases. Western blot was performed in order to find the target of miR-145.Results:MiR-145expression is under-regulated in human renal carcinomas, bladder tumors, renal caner cell lines and bladder cancer cell lines. miR-145suppresses invasion and metastasis in vivo.miR-145directly regulates N-cadherin in renal and bladder cancer cells; Furthermore, we found significantly decreased MMP9protein levels in cancer cell lines transfected with miR-145mimics.Conclusion:MiR-145suppresses tumor metastasis by inhibiting the protein translation of its direct target gene N-cadherin and indirect decreased MMP9protein levels. Also found that miR-145expression is under-regulated in human renal carcinomas, bladder tumors, renal caner cell lines and bladder cancer cell lines.