Effect Of Oat β-Glucan On Plasma Lipid And Antioxidant Ability In IGR Mice

Beta-glucan has been verified with lowering blood lipid, regulating blood sugar,enhancing immunity and other physiological effect. Impaired glucose regulation (IGR)is the prophase of diabetes status. The lipid level and the oxidation state of equilibriumare likely to change in this phase, during which disorders of lipid metabolism andoxidation balance may happen.This experiment researched the effect of oat β-glucan molecular weight and dosagein blood lipid and antioxidant ability of IGR mice. HFFD and10%fructose drinkingwater were uesd to induce IGR mice. Then IGR mice were used for the study of theeffect of oat β-glucan in plasma lipid and antioxidant ability.100mice were randomlydivided into normal group (NC) and model group (MC). Mice of NC group fed on abasic diet and ordinary drinking water. Mice of MC group fed on HFFD and10%fructose drinking water. After10weeks, the blood glucose level of mice in MC groupreached IGR standard, accompanying obesity and high cholesterol. After the modelestablishment, different molecular weights and dosage of β-glucan were used in thedietary intervention, blood samples were collected to determine the plasma totalcholesterol (TC), triglycerides (TG), high-density lipoprotein cholesterol (HDL-C) andlow density lipoprotein cholesterol (LDL-C) level at4th,7thand11thweek respectively.At the end of the test,the plasma free fatty acid (FFA), superoxide dismutase (SOD)activity, total antioxidant capacity (T-AOC) and malondialdehyde (MDA) contentwere determined as well as the coefficient of liver, spleen and kidney. The conclusionsare as follows:1. The weight, spleen coefficient and the coefficient of kidney of IGR mice werelower than the normal mice, there was no significant change in liver coefficient. Afterβ-glucan intervention, body weight and liver coefficient of IGR mice have nonoteworthy changes, there was an obvious rise in spleen and kidney coefficient, and thelower molecular weight of β-glucan had more noticeable effect on kidney coefficient.2. The IGR mice showed characteristics of high TC, LDL-C and elevated FFAlevels, there was no significant change in plasma HDL-C level, and plasma TG leveldecreased obviously. The different molecular weights and dosages of oat β-glucansignificantly reduced the TC and LDL-C levels of IGR mice, and the low molecularweight of β-glucan lowered the levels of serum TC and LDL-C effect more significantly;β-glucan recovered the level of plasma TG to normal, and high molecular weight of β-glucan showed a more significant effect, but there was no obvious effect on plasmaHDL-C level. The low molecular weight of β-glucan with high dose increased plasmaFFA level of IGR mice markedly.3. The IGR mice have lower plasma SOD and T-AOC than normal mice, but theMDA was significantly higher than normal mice; Oat β-glucan significantly improvedthe plasma SOD activity and T-AOC ability of IGR mice, and decreased MDA levelobviously. The ability of the low molecular weight of oat β-glucan in increasing theT-AOC level was more outstanding. And higher dose presented a better effect, whichshowed a dose-dependent manner.