Involving The Bone Of Limbs To Double Vertical Mixture Combined Improved Hulth Method Rat Model Of Knee Osteoarthritis Collagen X Aggrecan Effect Observation

Objective: to establish a kind of similar to human disease process to double Hulthforelegs vertical joint improvement method of KOA knee osteoarthritis in rats model,in this model, on the basis of success, involving the bone mixture on the modelCollagen X Aggrecan.Method: choose6only15days of age of SD rats during pregnancy, cage, the birthday48were only3, size sorted by weight were randomly divided into four groups,each group of12, only normal respectively all fours group to double fore upright todouble arms control to double fore joint Hulth group normal all fours do not make anyprocessing, the rest three groups respectively truncated double arms, raised in cagebreeding to double arm joint control: control, in March after surgery to double theirforelimbs joint Hulth group3months after surgery: surgery improved Hulth method,four groups after the second surgery in January, after death, deep anesthesia,respectively in four groups, each rat knee, HE dyeing red solid green dyeing intoluidine blue staining and immunohistochemical observation analysis. Based onevaluation model of success, to choose8only15days of age of SD rats duringpregnancy, cage, born to60were only3days, according to the weight size sortedrandomly divided into4groups, each group of15only, respectively to two forelegsvertical joint modified Hulth method of model group and normal saline groupsinvolving the bone mixture ammonia sugar beauty symplectic group in modifiedHulth method to double their forelimbs in April after surgery, four groups after the second surgery in January, after death, deep anesthesia were randomly selected fromthree in four groups to identify the model, after the success of the model identification,giving each corresponding drug intervention, involving the bone mixture group at1ml/100g weight for involving the bone mixture to fill the stomach; Ammonia sugarthe symplectic group press2mg/100g weight to give ammonia sugar beauty machineto fill the stomach; Physiological saline group at1ml/100g weight given salinelavage; Blank control group did not do any intervention treatment for4consecutiveweeks, deep anesthesia execution groups of rats, remove the knee, HE dyeingtoluidine blue staining Tibetan red solid green dyeing and immunohistochemicalobservation analysis.Results: the morphological analysis showed that normal all fours HE dyeing toluidineblue staining to hide red solid green display of articular cartilage surface smooth leveloff, the cartilage cell uniform distribution, sorting, clear at all levels to double foreupright set to double fore limbs joint control to double joint Hulth set of articularcartilage surface showed ulcer or cracks, and even local defects, cartilage cellsdecreased significantly, the cartilage layer of uneven dyeing, partial failures dyeingphenomenon; Articular cartilage cells in each layer a cluster sample, growing deepmast cartilage cells; Especially in the limbs to double joint Hulth group was mostpronounced by groups of drug intervention, and to double modified Hulth forelegsvertical joint model group and normal saline group, involving the bone mixtureammonia sugar mei xin group of articular cartilage surface smooth level off, the jointsurface is continuous, cartilage cells are uniformly distributed and sorting is neat, atall levels is clear, no obvious cell cluster phenomenon, tide line complete, partialdyeing is homogeneous.Conclusion: successfully established to double fore upright to double forelegsvertical joint control to double forelimbs upright Hulth a combination of three models,including double Hulth forelegs vertical joint improvement method to more typicalmodel, this model is simple, strong repeatability, in order to further study the pathogenesis of osteoarthritis and development laid the foundation for the furthereffective treatment measures involving the bone mixture can delay to erect doubleforelimbs modified Hulth method for knee joint osteoarthritis joint cartilagedegeneration model state, can be adjusted via increased extracellular matrix Aggrecanexpression Collagen X expression, delay the degeneration of articular cartilage ofknee joint process.