Protective Effects Of Vitamin D₃on Acute Liver Injury Induced By CCL4in Mice

Objective:To explore the protective effects of vitamin D3on CCl4induced acute hepatic injury in mice.Methods:Forty three Kunming mice were randomly and equally divided into five groups:normal group, model group, and three vitamin D3groups. Each group was fed a normal diet. On the basis of the liver injury model established by0.1%CCl4solution of olive oil(0.1ml/10g/2d,ip).The normal group and model group give daily intraperitoneal injection of0.9%Nacl and three treating groups of vitaminD3give intraperitoneal injection once everyday. Doses of vitamin D3in each group are5ug,2.5ug.1ug. At the end of the7th day, eyes blood and liver tissue samples were taken from all the mice. Serum biological parameters were determined routinely, and pathological changes in liver tissues were detected by HE staining.Results:The levels of alanine aminotransferase, aspartate transaminase were lower significantly in the high-dose vitamin D3group than in the model group (all P <0.01,), Mice of the model group showed moderate fatty liver degeneration and necrosis, and varying degrees of improvement of these hepatic pathologic lesions were observed in the vitamin D3groups.Conclusions:These results suggest that vitamin D3had curative effects on liver injury in mice induced by CC14.