| Objective:Through clinical observation of homemade jade red cream in promoting postoperative wound healing of anus week abscess of the clinical efficacy and safety; Through the animal experiment to observe self-made jade red cream wound granulation tissue of rat effect of exogenous epidermal cell growth factor expression.Method:Clinical research:70cases will be included in the standard case, were randomly divided into treatment group and control group,35cases, take a random number table method classification controlled clinical study.3days for a period of treatment, drug use after3days, after7days,10days later, after15days,20days after each record1clinical curative effect). Of drug use before and after3,7,10,15,20days to compare postoperative granulation tissue growth degree, the degree of pain of the wound, wound secretion stimulation. Hospitalization days, hospitalization days will be subject to20days or so), using VAS score standard, the first day after the treatment group and control group for3days (Ⅱ first-generation cephalosporin antibiotics therapy, fat-soluble vitamin injection support, since the first day postoperatively, the treatment group daily routine dressing on the wound, with homemade jade red cream daub gauze to cover. Daily routine dressing change was carried out on the wound and the control group, with ma yinglong muskiness daub ointment of gauze to cover, to compare the curative effect of treatment group and control group.Experimental study:the experiment is divided into three groups, homemade jade red cream group of musk, ma yinglong haemorrhoid creams and vaseline group. Select10%chloral hydrate, according to the concentration of300mg/kg anesthesia model rats. After shave Mao Bei back local skin and disinfection, use the punch in30Wister rat dorsal (on the left side of the spine) hole, preparation of diameter is1.8cm, covers an area of2.54cm squared circle of full-thickness skin ulcer models, respectively, with this three kinds of drug treatment, the treatment after3,7,14,21days dynamic contrast the wound granulation tissue was observed, the content of exogenous epidermal growth factor in to discuss homemade jade red cream promotes wound healing mechanism.Results:Clinical research:(1) the total cure rate94.29%treatment group, control group cure rate82.86%.(2),(35.89+14.16) treatment group and control group (37.09+12.84) age composition were no significant differences by statistical analysis (P>0.05); Pain scores compared the two groups after treatment (3) there are significant differences,15days after the control group (2.51+1.12)(1.60+0.81) pain obviously in the treatment group, control group20days postoperatively (1.94+0.59)(1.20+0.99) pain obviously in the treatment group, treatment group was superior to control group (P<0.05); Granulation tissue growth situation to compare the two groups after treatment had significant difference, the treatment group (1.66+0.54)10days postoperatively compared with the control group (2.11+0.40) granulation growth obviously,15days after the treatment group than the control group (1.37+0.55)(1.77+0.81) granulation growth obviously, after20days of treatment group than the control group (0.37+0.77)(0.89+1.35) granulation growth significantly, the treatment group is better than that of control group (P<0.05); Two groups after treatment on the surrounding conditions to stimulate the skin wound secretion have significant difference, the control group after3days (2.57+0.92)(2.17+0.57) in the treatment group stimulate skin secretion, control group (2.86+1.22) for7days after surgery (2.11+0.47) in the treatment group stimulate skin secretion, control group (2.74+1.29)10days postoperatively (1.71+0.71) in the treatment group stimulate skin secretion,15days after the control group (2.69+1.28)(1.89+0.68) in the treatment group stimulate skin secretion,20days after the control group (2.34+0.91)(1.09+1.01) in the treatment group secretions to stimulate the skin, the treatment group is better than that of control group (P<0.05); Two groups after treatment, WBC, neutrophil comparative has significant difference,10days after the treatment group (white blood cells:7.22+/-1.37x109, neutral:59.17%+/-6.60) than the control group (white blood cells:8.95+/-0.94x109, neutral:67.51%)4.32mm white blood cells and neutrophils significant difference, the treatment group is better than that of control group (P<0.05),(4) in treatment group were treated by self-made jade red cream promote postoperative wound healing of anus week abscess has good promoting effect.Experimental study:homemade jade red cream with ma yinglong musk hemorrhoids ointment has obvious promoting effect to wound healing in rats,3and7days after drug healing rate has no obvious difference between the three groups, P>0.05; In14days after the medication, jade red cream group compared with the other two groups have significant difference, P<0.05, jade red cream wound healing rate was significantly higher than the other two groups, ma yinglong and vaseline group compared with significant difference, P<0.05, ma yinglong healing rate is higher than vaseline group; In21days after the medication, jade red cream group compared with the other two groups have significant difference, P<0.05, jade red cream wound healing rate was significantly higher than the other two groups, ma yinglong group compared with vaseline group, the healing rate was comparable (P>0.05); Wound granulation tissue EGF concentrations in3days, no significant differences between groups, P were greater than0.05;7day, jade red cream with ma yinglong group comparison, P=0.334>0.05, jade red creams and vaseline group comparison (P=0.007<0.05, ma yinglong and vaseline group comparison (P=0.187>0.05. Jade red cream is better than that of vaseline group, compared with ma yinglong group have no obvious difference;14days, jade red cream with ma yinglong group comparison (P=0.037<0.05, jade red creams and vaseline group comparison (P=0.035<0.05, jade red cream group better than the other two groups, ma yinglong and vaseline group was no significant difference (P=0.273>0.05;21days, jade red cream with ma yinglong group comparison (P=0.020<0.05, jade red creams and vaseline group comparison (P=0.001<0.05, ma yinglong and vaseline group was no significant difference (P=0.413>0.05, homemade jade red cream group was better than other two groups, can significantly improve wound granulation tissue EGF concentrations, accelerate wound healing. Conclusion:Homemade jade red cream may enhance the EGF concentrations in the granulation tissue, achieve the purpose of promoting wound healing, the postoperative wound healing of anus week abscess has significant differences in pain sensitivity, the treatment group after the article packed homemade jade red cream sand, granulation tissue growth than the control group obviously accelerated, its on the surrounding skin wound secretion stimulation significantly reduce, blood cell analysis is improved significantly before, thus demonstrates the homemade jade red cream in promoting nest crissum abscess postoperative wound healing has obvious curative effect, and easy to take, method is fast and effective, the patient easy to accept, worth clinical promotion.This topic through the clinical and experiment two parts, for the clinical curative effect of homemade jade red cream promote wound healing, mechanism provides certain scientific basis. |
PDF Full Text Request