MicroRNAs Expression Profile Analysis In The Mice With Alcoholic Cardiomyopathy

Chronic excessive alcohol intake may lead to progressive and chronic cardiac dysfunction and can be a possible cause of dilated cardiomyopathy, referred to as alcoholic cardiomyopathy. The incidence increased year by year at the moment, and the harm to us is serious, but the mechanism of action of microRNAs in alcoholic cardiomyopathy is not clear. In this study, the variation of microRNA expression in normal and alcoholic cardiomyopathy cells was found by microarray analysis. Combining with bioinformatics prediction, the clues of microRNAs involving in alcoholic cardiomyopathy was studied. It was found that the microRNAs play a regulation role in cardiac hypertrophy by research methods for microRNA molecular biology. The theoretical basis for illustrating the function of microRNA in cardiac development, hypertrophy, arrhythmia, myocardial fibrosis, heart failure and other heart function in physiological and pathological processes was provided. It may be the new idea and strategy for the prevention and treatment of cardiovascular diseases in the future.Research Purposes:(1) To establish model of mice with alcoholic cardiomyopathy by feeding alcohol method;(2) To screen alcoholic cardiomyopathy miRNAs related to myocardial tissue lesions in mice with the use of microarray, and to find out molecular basis of alcoholic cardiomyopathy;(3) To confirm the differentially expressed miRNAs by using Real-Time PCRMethodsFifty male Kunming mice, weighing15-20g, were randomly divided into control group (n=20) and alcoholic cardiomyopathy experiment group (n=30). All animals were provided by Animal Center of Kunming Medical University. Mice in experiment group have been fed with4%ethanol for12weeks, mice in the control group with equivalent amount of normal saline. We took out hearts to make pathological sections after the model was successfully established. We observed morphological changes in myocardial cell under an optical microscope following HE staining. After twelve weeks’ feeding, the hearts were taken out, total RNA was extracted by Trizol Method and the differential expression of microRNA was quantitatively detected by microarray and miRNA fluorescence quantitative PCR kit. Research results(1) Compared with control group, the hearts of experiment group was not significantly greater than those of the control group. However, significant change was found in the left ventricle, ventricular septal. Pathological findings showed that myocardial cells in experiment group were greater than those in control group.(2) The expression of mmu-miR-X1, mmu-miR-X2, mmu-miR-X3in the alcoholic cardiomyopathy experiment group was significant higher than that in control by Microarray analysis and Real-time PCR. However, The expression of mmu-miR-X5, mmu-miR-X6, mmu-miR-X7, mmu-miR-X8, mmu-miR-X9, mmu-miR-X10, mmu-miR-X11, mmu-miR-X12was lower than that in control group。Conclusion(1) Myocardial tissue with alcoholic cardiomyopathy in mice compared with normal cardiac tissue, the miRNAs were differentially expressed. (2) The miRNAs associated with myocardial tissue lesions with alcoholic cardiomyopathy were mmu-miR-X1, mmu-miR-X2, mmu-miR-X3, mmu-miR-X5, mmu-miR-X6, mmu-miR-X7, mmu-miR-X8, mmu-miR-X9, mmu-miR-X10, mmu-miR-X11, mmu-miR-X12。...