The Study Of Relationship Between Alcoholic Cardiomyopathy And CYP2E1DraI Gene Polymorphism

Objective: Alcoholic cardiomyopathy refers to cardiomyopathy caused by long-term heavy intake of alcohol.Previous studies have suggested that genetic factors may play a vital role in the nosogenesis of alcoholic cardiomyopathy.Cytochrome P4502E1(CYP2E1),as a typical etho-induced enzyme,is involved in the metabolism of alcohol in vivo.Among them,Dra I polymorphism is the most common.In this study,the polymorphism of Dra I locus of CYP2E1 gene was detected to explore its relationship with the incidence of alcoholic cardiomyopathy,so as to provide the basis for the diagnosis and management of alcoholic cardiomyopathy.Methods: In this case-control study,male patients with alcoholic cardiomyopathy who were hospitalized in the Department of Cardiology,Affiliated Hospital of Jining Medical University from June 2020 to December 2022 and healthy adult males who underwent medical examination in a health examination centre during the identical stage were chosen as the research objects.According to whether they had alcoholic cardiomyopathy and whether they were addicted to alcohol,They were divided into healthy control group,alcoholic cardiomyopathy group and alcoholic group without cardiomyopathy with 80 cases in each group.Clinical data of the subjects were collected using the nori electronic medical record system and correlation analysis was carried out.5ml fasting peripheral venous blood of the subjects was collected and Polymerase Chain Reaction was used.The genotype and allele of CYP2E1 Dra I were determined by PCR and next generation sequencing.Analysis of the differences in allele and genotype frequencies between the three groups of samples,and P < 0.05 was deemed to be statistical significance.Results:Through the analysis of the baseline data of the three groups,it was found that the BMI,blood uric acid and blood glucose levels of the alcoholic cardiomyopathy group and the alcoholic group without cardiomyopathy were much than those of the healthy matched group(P <0.05).CYP2E1 Dra I gene was consistent with Hardy-Weinberg(HWE)balance law,and the distribution of CC,CD and DD genotypes in alcoholic cardiomyopathy group was as follows: 40 cases(50%),29 cases(36.25%),11 cases(13.75%),and the distribution of three genotypes in alcoholic group without cardiomyopathy was as follows: There were 38cases(47.5%),34 cases(42.5%),8 cases(10%),and 63 cases(78.75%),14 cases(17.5%),3 cases(3.75%)of the three genotypes in the healthy matched group.Frequencies of CD and DD genotypes in the alcoholic cardiomyopathy group and the alcoholic group without cardiomyopathy was much than that in the matched group(P < 0.05).The frequency of C(D)allele in alcoholic cardiomyopathy group,alcoholic group without cardiomyopathy and healthy matched group was 68.1%(31.9%),68.8%(31.2%)and 87.5%(12.5%),respectively.Frequencies of D allele in alcoholic cardiomyopathy group and alcoholic group without cardiomyopathy was obviously much than that in healthy matched group(P < 0.05).Frequencies of C allele were obviously less than that of healthy matched group(P < 0.05).There was no prominent discrepancy in frequency distribution of the three genotypes between alcoholic cardiomyopathy group and alcoholic group without cardiomyopathy,and there was no prominent discrepancy in allele frequency(P > 0.05).Conclusion:This study showed that the polymorphism of Dra I locus(rs6413432)of CYP2E1 gene was connected with alcohol dependence in the Han population in southwest Shandong Province,China.The mutant allele D of this locus is a susceptibility gene for alcohol dependence,and its relationship with alcoholic cardiomyopathy has not been confirmed.