| OBJECTIVE: To investigate the changes of echocardiography,laboratory parameters and histopathology in the process of alcoholic cardiomyopathy after withdrawal from alcohol by establishing a model of alcoholic cardiomyopathy in rats and treating alcohol withdrawal.METHODS: A total of 112 male clean-grade SD rats were divided into 80 model groups and 32 normal controls.They were intraperitoneally administered by alcohol and aminotriazole(a specific catalase inhibitor).Injection,daily drinking water containing 10% alcohol for a total of ten weeks,establish ACM model.The modeled ACM model group was then randomly divided into alcohol group(n=35)with continued alcoholic administration and alcoholic group(n=33)with equal saline,and firstly after modeling.Echocardiography,biochemical indicators were measured after month,after the second month and after the third month and compared with pathological results.RESULTS: Echocardiographic index: In the first month after alcohol withdrawal,there was no significant change in the morphological indexes such as IVSDd,LVEDd and LVPWDd in the first week after the model was completed(P>0.05).The EF and FS of cardiographic function indicators were increased(P<0.05).The second and third months after abstinence,the alcohol-dissipating group began to increase IVSDd and LVPWDd,and LVEDd began to shrink.EF,FS also gradually increased(P<0.05);there was no significant change in heart rate between the alcohol group and the alcohol withdrawal group(p>0.05).Biochemical indicators: In the first month after alcohol withdrawal,CK,CK-MB,Hs-TnI,Nt-proBNP,and LDH in the biochemical indicators of alcohol withdrawal group were lower than those in the tenth week after modeling(P<0.05).However,Hs-CRP increased compared with the tenth week of modeling(P<0.05);in the second month after alcohol withdrawal,Hs-CRP in alcoholics group decreased compared with the first month after alcohol withdrawal(P< 0.05);Hs-CRP in the alcohol withdrawal group was higher than the control group(P<0.05),and in the third month after alcohol withdrawal,the CK,CK-MB,and Nt-proBNP in the alcohol withdrawal group were not different from the control group.Statistical significance(P>0.05),Hs-CRP in the alcohol withdrawal group was higher than the control group,the difference was statistically significant(P<0.05).Pathological control: After abstinence,the myocardial cell size of the rats in the alcohol withdrawal group was similar to that of the control group,the cytoplasm was relatively uniform,and the myocardial cells were arranged neatly.At the third month after alcohol withdrawal,the myocardial fibers arranged slightly wavy.The normal control group was similar;while the number of cardiomyocytes in the alcohol group continued to decrease,the cell morphology was abnormal,diffuse degeneration,fibrosis,a large number of small vacuoles were observed in the cells,and the cell nuclei shrinked.A large amount of fat is accumulated,and a large number of inflammatory cells are also aggregated.Conclusion: 1.Alcoholic cardiomyopathy is a reversible myocardial disease.2.Echocardiography and myocardial damage markers can be used to assess the natural outcome of alcoholic cardiomyopathy in rats.3.High-sensitivity C-reactive protein can be used to assess acute alcohol damage in rats,but it cannot be used to assess the natural outcome of chronic alcoholic cardiomyopathy in rats.4.Amino terminal brain natriuretic peptide can be used to help assess the natural outcome of alcoholic cardiomyopathy in rats.5.Histopathology can be used to help assess the natural outcome of alcoholic cardiomyopathy in rats. |
PDF Full Text Request