| ObjectivesTo investigate influence of cyprinus carpio decoction on integrin-linked kinase (ILK) signal pathway reducing nephrotic syndrome (NS) rats urine protein and preventing epithelial-mesenchymal transition.Methods50Male Wistar rats were randomly assigned to5groups:control group (group N), adriamycin nephropathy group (group S), high cyprinus group (group H), low cyprinus group (group L) and Irbesartan group (group IR), which all rats except control group received ADR6.2mg/kg via injecting from vena caudlis, to introduce ADR induced nephropathy. Rats of the control group were injected with normal saline via the tail vein. Then begin the treatment one week later. Rats in group H and group L receive CCD by lavage; and group IR receive Irbesartan by lavage. Rats in group N and group S are given the same amount of water. The serum biochemical values of the total protein (TP), albumin (Alb), blood urea nitrogen (BUN), serum creatinine (SCr), cholesterol (CHO), triglycerides (TG) and the urinary protein (UP) content in12h urine were measured. ILK and phosphorylation of glycogen synthase kinase-3β (P-GSK-β) expression, β-catenin nuclear positive rate, a-smooth muscle protein expression intensity (a-SAM) in renal tissues were determined by immunohistochemical assay.Results(1) Urinary protein excretion in12h urineBefore injection of ADR, there is no significant difference among all rats (P>0.05). After injection of ADR, the value of Urinary protein excretion in12h urine is gradually increasing. And rats receiving injection of ADR is higher than that of rats in group N. Although Urinary protein excretion in12h urine of the rats received Irbesartan and CCD was higher than that of the normal rats (P<0.05), it was less than group S since the second week after injection of ADR. And since the ninth week, the difference is significant (P<0.05).(2) Biochemical indicator Administration of CCD and Irbesartan significantly prevented the decrease in serum albumin (.P<0.05, compared with group S), but the differences between group H/L and group IR. Concentrations of TG and CHO in serum of rats receiving injection of ADR are higher than group N (P<0.05). The level of TG in group H/L is lower than group S, but no differences are found in group IR. The level of SCr in group H, group IR, group S is higher than group N (P>0.05), but no differences are found among these rats. There was no significant difference in serum SCr concentration among group S, group H, group L, group IR (P>0.05).(3) Renal tissue morphological analysisThe group N rats glomerular and tubular interstitial no change. The rats of group S glomerular epithelial cell enlargement, mesangial proliferative wider matrix and tubular epithelial cell vacuoles, tubular atrophy, dilation, the lumen a lot of protein tube type, diffuse inflammatory cell infiltration, interstitial fibrosis were obsevered. However, group H, group L, group IR is better improve than group S.(4) ImmunohistochemistryBrown or yellow granular mass, in a continuous shape of thick line, was found in the tubular, in each group. The expressions of ILK, P-GSK-3β, β-catenin, a-SMA in renal tissues of ADNs were increased with comparsion of that in normal group, especially the model group the hightest. In the rats received Irbesartan and CCD, the area distribution was less than that in group S (P<0.05, compared with group S).ConclusionIn conclusion, the present study shows that CCD has a protective effect against ADR-induced nephropathy, this effect may be attributed to reduce proteinuria and elevated12h total volume of urine and serum albumin.Moreover, the roles of CCD are related to the expression of ILK/GSK-3β/β-catenin and a-SMA, and CCD can prevent EMT. |
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