| PurposeGlomerular extracellular matrix (ECM) synthesis and degradation of the imbalance can lead to the barrier function of the glomerular filtration membrane does,esulting in proteinuria and the development of fibrosis and even hardening of matrix metalloproteinase-2(MMP-2) and its inhibition factor TIMP-2in the regulation of the ECM metabolism, as well as by the regulation of TGF-β1. This study was to preparation of the nephrotic syndrome model rats with adriamycin. carp soup preparations to intervene to observe the expression of MMP-2and TIMP-2, TGF-β1in the kidney tissue expression and carp soup. its expression, while observing the changes in urinary protein excretion,blood chemistry and pathology. Explore the protective effect and mechanism of the carp soup for nephrotic syndrome in rat kidney.Method1. Preparation of animal models and intervention:50Wistar rats were adaptive feeding for a week, urinary protein tested negative were randomly divided into the group N, group S, group IR, group L (n=10), group H (n=10). Reference to the method of Bertani In addition to the group N rats in each group, giving a single dose of intravenous injection of doxorubicin6.2mg/kg, group N rats was given a one-time intravenous injection of normal saline.After injection, group H were intervented with500%carp soup9ml/kg, group L were intervented with250%carp soup9ml/kg (equivalent to70kg normal adult dose calculation,in accordance with the rat and human body surface area conversion coefficient of0.018); group IR to50mg/kg gavage (dissolved in the drinking water9ml/kg); group S,N drinking water9ml/kg orally once a day, a total of intervention8w.2. Biochemical tests:urine specimens using metal metabolism cages to collect, fasting can not help the water to collect the rats injected with doxorubicin before and after injection of adriamycin,1w,2w,3w...10w morning urine and12h urine were measured12h urinary protein. Experiment10weeks take heart blood,determination of total protein, albumin,creatinine, urea nitrogen, cholesterol, blood lipids. 3. Pathology:conventional paraffin sections,HE staining under the light microscope to observe the morphological changes, IPP6.0professional image analysis software to calculate the glomerular cross-sectional area,glomerular volume.4. Immunohistochemical detection of MMP-2, TIMP-2protein, TGF-β1of expression:SABC immunohistochemical staining the IPP6.0image analysis software sections immunohistochemical results, the calculation of the average optical density.Result1.12h urinary protein:repeated measures analysis of variance results show that the modeling groups’12h urinary protein excretion difference was not statistically significant;one week after modeling,the group S, group L, group H, group IR12h urinary protein excretion was statistically significant (P＜0.05) compared with the N showed that the model was successful;since eventh week,the group L, group H, group IR12h urinary protein excretion the amount was the time of dependence, the10th week of experiments,the group L, group H, group IR12h urinary protein excretion was significantly lower (P＜0.05),compared with the group S difference was statistically significant (P＜.05).2. Blood biochemical indices:the group N, the group S rats TP, ALB, significantly reduced (P＜0.05), Cr, BUN, a significant rise (P<0.05).Compared with the group S, group L, group H improved significantly (P＜0.05).3. Pathological findings:group N glomerular size, open capillary loops, inflammation, invasion, and ECM accumulation; group S rats glomerular volume increased significantly (compared with the group N, P＜0.05),capillary loops change stenosis or occlusion of a large number of inflammatory cell invasion and increased mesangial cells, glomerular cells decreased,showing that glomerular fibrosis; group L, group H, slight increase in the IR glomerular volume,mesangial cell increase was not obvious, part of the glomerular capillary lumen stenosis,inflammatory invasion is not obvious.(Figure1).4. The immunohistochemical staining results:compared to the group N, group S rats kidney tissue expression of MMP-2and TIMP-2protein decreased, expression of TGF-β1was significantly upregulated (P＜0.05) expression in the glomerular matrix,tubulointerstitial; compare with the group S, group L, group H, group IR rats kidney tissue MMP-2and TIMP-2protein expression increased and TGF-β1expression decreased (P<0.05),MMP-2/TIMP-2in value significantly1high (P＜0.05).ConclusionCarp soup in increased serum albumin, ower proteinuria aspects have a significant effect by regulating the ECM degrading enzymes activity, lower of TGF-β1expression, increase MMP-2and TIMP-2expression, increasing to reduce ECM protein synthesis, and promote its degradation,mitigate the proliferation of mesangial cells and matrix,reducing the inflammatory response, to achieve remission of renal fibrosis and to improve the role of renal function. |
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