The Study Of The Astragalus On The Apoptosis Cellular Morphology And Apoptosis-related Proteins And Their Genes Role Surround The Focus Of Cerebral Hemorrhage In Rats

Objective To observe the study of the Astragalus on the apoptosis cellular morphologyand apoptosis-related proteins and their genes role surround the focus of cerebralhemorrhage in rats, and explore the laws of the cellular apoptosis and the possibleapoptotic pathway after intracerebral hemorrhage in rats, theoretical and experimentalbasis for Astragalus used in the clinical treatment of cerebral hemorrhage.Method240healthy adult male SD rats, weighing300±50g. Electron microscopy,immunohistochemistry, RT-PCR, each with the mouse80, each divided into normalgroups, model groups, Astragalus injection groups and Astragaloside IV groups, eachsubdivided into1d,2d,3d,7d group, each group of5. Heparinized IV collagenase wereinjected into rats caudate nucleus to create cerebral hemorrhage model by stereotaxicinstrument. Each drug group SD rats after cerebral hemorrhage modeling wereintraperitoneal injection of Astragalus injection (4g^-1·d^-1) or Astragaloside IV （4mg kg-1d-1） intervention on0h, followed by injection every24h. Normal group and model grouprats were injected intraperitoneally corresponding amount of saline. Respectively byneurological behavior score, electron microscopy, immunohistochemistry and RT-PCR toobserve the group rats neuroethology of perihematomal, neuron ultrastructure changes,apoptosis-related protein expression and related apoptosis gene expression, which explorepossible pathways of cellular apoptosis after cerebral hemorrhage in rats, some experim-ental evidence for Astragalus used in the clinical treatment of cerebral hemorrhage. Results①Rats neurological behavior score: Astragalus injection groups andAstragaloside IV groups could significantly reduce the level of intracerebral hemorrhagein rats nerve function behavior scores（P<0.05）. Astragalus injection7d groups and theAstragaloside IV7d groups neurobehavioral recovery were significantly better than thecorresponding1d,2d,3d treatment group（P<0.05）. Compared with Astragalus IV3d,7dgroup, Astragalus injection corresponding group neurological behavior score wassignificantly lower（P<0.05）.②Electron microscopy: cellular ultrastructure injurysurround focus of intracerebral hemorrhage in rats of Astragalus injection andAstragaloside IV3d and7d group was significantly lighter than the model3d,7d group.Cell ultrastructure recovery of Astragalus injection and Astragaloside IV7d group wasbetter than Astragalus injection and Astragaloside IV3d group. Cell ultrastructurerecovery of Astragalus Injection7d group was better than Astragaloside IV7d group.③Immunohistochemical staining of rat brain tissue: Bcl-2, Bax and Caspase-3in thenormal brain tissue were only a small amount of expression and Bcl-2/Bax ratio close to1.Compared with the normal group, model group, Bcl-2, Bax and Caspase-3protein positiveexpression of Astragalus injection group and Astragaloside IV group were significantlyincreased（P<0.05）. Compared with the corresponding2d,3d,7d model group, Bcl-2protein expression of Astragalus injection and Astragalus IV2d,3d,7d group wassignificantly increased（P<0.05）, Bax and Caspase-3protein positive expression weresignificantly lower（P<0.05）. Compared with Astragalus injection and Astragaloside IV3dgroup, Bcl-2protein positive expression of Astragalus injection and Astragaloside IV ineach7d group was significantly increased（P <0.05）, Bax and Caspase-3protein positiveexpression were significantly lower（P<0.05）. Compared with Astragalus IV3d group,Bcl-2protein positive expression of Astragaloside injection3d group was significantlyincreased （P<0.05）, Bax and Caspase-3protein positive expression wre significantly lower（P<0.05）. Compared with Astragalus IV7d group, Bcl-2protein positive expressionof Astragaloside injection7d group was significantly increased （P<0.05）, Bax andCaspase-3protein positive expression wre significantly lower（P<0.05）.④RT-PCR: Bcl-2mRNA, Bax mRNA and Caspase-3mRNA in the normal brain tissue were only a smallamount of expression and Bcl-2mRNA/Bax mRNA ratio close to1. Compared with thenormal group, Bcl-2mRNA and Bax mRNA and Caspase-3mRNA expression of modelgroup and Astragalus injection group and Astragaloside IV group were significantlyincreased（P<0.05）. Compared with the corresponding2d,3d,7d model group, Bcl-2mRNA expression of Astragalus injection and Astragalus IV2d,3d,7d group wassignificantly increased（P <0.05）, Bax mRNA and Caspase-3mRNA expression weresignificantly lower（P<0.05）. Compared with Astragalus injection and Astragaloside IV3dgroup, Bcl-2mRNA expression of Astragalus injection and Astragaloside IV7d groupwas significantly increased（P<0.05）, Bax mRNA and Caspase-3mRNA expression weresignificantly lower（P<0.05）. Compared with Astragalus IV3d group, Bcl-2mRNAexpression of Astragaloside injection3d group was significantly increased （P<0.05）, BaxmRNA and Caspase-3mRNA were significantly lower（P<0.05）. Compared withAstragalus IV7d group, Bcl-2mRNA expression of Astragaloside injection7d group wassignificantly increased（P <0.05）, Bax mRNA and Caspase-3mRNA were significantlylower （P<0.05）.Conclusion①Neurological behavior and the ultrastructure of apoptotic cell surroundthe focus of intracerebral hemorrhage in rats could be improved to varying degrees byAstragalus.②Cell apoptosis surround the focus of intracerebral hemorrhage in ratscould be inhibited to varying degrees by Astragalus. Its mechanism could be through theenhancement Bcl-2mRNA transcription and weakening Bax mRNA, Caspase-3mRNAtranscription, and increased Bcl-2protein synthesis and decreased Bax, Caspase-3 protein synthesis, which in turn inhibits neuronal apoptosis surround the focus ofintracerebral hemorrhage in rats.③With the extension of the medication time, Theinhibition of cellular apoptotic surround the focus of intracerebral hemorrhage in rats byAstragalus is more pronounced, and the inhibition of Astragalus injection is better thanthat of Astragaloside IV.