The Preliminary Study Of The Relationship Between ELSPBP1Gene And Lyzl Gene Family And Reproduction

With the social progress and growth in the living standard, the topic of prenataland postnatal care has been concerned gradually. Spermatogenesis and anacmesisfactors lead to the incidence of male infertility on the rise. We studied ELSPBP1geneand Lyzl gene family closely related to spermatogenesis in this project.Objectives Explore the expression characterization and potential function ofthe the gene ELSPBP1and Lyzl gene family in the reproductive.Methods The human tissues mRNA was extracted, the gene expression wasdetected by RT-PCR. Also the differential expression of ELSPBP1in various segmentof epididymal duct was detected by RT-PCR. By immunohistochemical stainingmethod to determine the cellular location of the gene in the human testis andepididymis. The positioning of ELSPBP1in human ejaculated spermatozoa wasdetermine by immunofluorescence. The expressions of Lyzls in mouse tissues anddifferent-week-old mice testis as well as epididymis was detected by RT-PCR.Building castration model and supplying physiological doses of testosterone, theexpression of Lyzl6in epididymis was detected by RT-PCR. Immunofluorescenceassay was explored to detect Lyzl6positioning changes in mouse sperm maturationprocess. The bacteriolytic activity of rLYZL6was analyzed by a colony-formingassay.Results Human ELSPBP1gene was expressed specifically in the testis andepididymis. The caput showed the highest expression level in epididymis, cauda was minimum level. Positive signals were on spermatocytes cytoplasmic, roundspermatids, elongated spermatids and principal cell cytoplasmic. ELSPBP1proteinwas located on the equatorial segment. All Lyzls were expressed at a higher level inthe testis and epididymis than in other tissues. There was a moderate expression ofLyzl1, Lyzl3, and Lyzl4in brain and lung, and Lyzl6was expressed slightly in theseminal vesicle, vas deferens, kidney and spleen. In the testis, all lyzls were expressedfrom day21during postnatal development. In the epididymis, a tenuous expression ofLyzl1existed at day28, and the highest expression was at day35. Lyzl3was slightlyexpressed on the14thand21thpost-natal days, and the expression was sharplyincreased by the28thday. On the28thday, the expression of Lyzl4, and Lyzl6were firstdetected, and high expression was maintained to the35thday. Lyzl4expression wasregulated by androgens in epididymis. Immunodetection revealed the presence ofLyzl6protein in primary spermatocytes and round spermatids and on thepost-acrosomal area and midpiece of mature spermatozoa. The rLYZL6proteinexhibited antibacterial activity against S. au.Conclusions Our results suggest ELSPBP1was expressed specifically in thetestis and epididymis, it may play important roles in spermatogenesis,sperm-maturation and sperm-oocyte membrane fusion. Lyzls were expressed in theepididymis and testis in a developmentally-regulated manner and one (Lyzl4) wasregulated by androgens. LYZL6may contribute to the native immunity of the malegenital tract.