Effect Of Guizhou Liquor On The Expression Of StAR、EGF、CgA And The Level Of Serum Testosterone In Rat Leydig Cell

Objective: To explore the consumption of a brand of Guizhou liquor(54 degrees and 38 degrees), the different time and different doses of rat Leydig cells within the Steroidogenic Acute Regulatory Protein(St AR)、 Epidermal growth factor(EGF)、Chromogranin A(Cg A)protein expression and the change of the serum testosterone level, so as to provide basic data for scientific and healthy drinking. Methods: The normal adult male SD 123 rats were randomly divided into Normal control group and Experimental group. 54 degrees and 38 degrees of the experimental group with 54 rats in each group were divided into 4w group, 8w group and 12 w group. Each group of rats were divided into Low dose group( L dose, 0.8ml/kg·d), Middle dose group( M dose, 1.6ml/kg·d) and High dose group( H dose, 2.4ml/kg·d). Each week there are 5 differences between the three groups of SD rats as normal control. The experimental group rats were given corresponding dose of liquor gavage daily, 11:00 Am and 5:00 PM respectively by gavage once a day prior to the sampling, the normal control group feeding. At the end of the 4th, 8th and 12 th week, the serum was collected and testicular tissue was taken. Using immunohistochemical SABC method, Image analysis, Western-blotting, Chemiluminescence method was studied. Results: 1. 54° group:(1) Chemiluminescence detection results of serum chemistry: No comparison of serum testosterone levels in each experimental group and normal control group was significantly abnormal, no statistically significant difference(P>0.05).(2) The results of immunohistochemistry SABC method: Under the light microscope, the St AR, EGF, Cg A immunohistochemical of positive products were brown or brown finely granular distribution in testicular Leydig cell cytoplasm. ?Compared with the normal control group, 4w, 8w and 12 w each dose group St AR staining intensity and average optical density increased(P<0.05). ?Compared with the normal control group, 4w, 8w and12 w each dose group EGF staining intensity and average optical density had no significant change(P>0.05). ?Compared with thenormal control group, 4w, 8w and 12 w each dose group Cg A staining intensity and average optical density had no significant change(P>0.05).(3)Western blotting results: ?4w, 8w and 12 w each dose group of St AR protein expression levels were significantly increased(P<0.05). ?4w, 8w and 12 w each dose of EGF protein expression levels had no significant change(P>0.05). 2. 38° group:( 1)Chemiluminescence detection results of serum chemistry: No comparison of serum testosterone levels in each experimental group and normal control group was significantly abnormal, no statistically significant difference(P>0.05).(2)The results of immunohistochemistry SABC method: ?Compared with the normal control group, 4w, 8w and 12 w each dose group St AR staining intensity and average optical density increased(P<0.05). ?Compared with the normal control group, 4w, 8w and 12 w each dose group EGF staining intensity and average optical density had no significant change(P>0.05). ?Compared with the normal control group, 4w, 8w and 12 w each dose group Cg A staining intensity and average optical density had no significant change(P>0.05).(3)Western blotting results: ?4w, 8w and 12 w each dose group of St AR protein expression levels were significantly increased(P<0.05). ?4w, 8w and 12 w each dose of EGF protein expression levels had no significant change(P>0.05). Conclusion: In this experiment a set dose and schedule after gavage with the liquor( 54° and 38°), serum testosterone levels did not change significantly.