Effect Of Tacrolimus On The Expression Of MIP-1α And IL-2on Autoimmune Prostatitis In Rat Model

Objective To investigate the role of MIP-1α and IL-2in the experimental autoimmuneprostatitis（EAP） rat model and study the efficacy of tacrolimus in treating EAP in rat modelas well as EAP’s immune mechanism and immunotherapy, which will provide a new pathway forthe clinical treatment of CP/CPPS.Methods1.The expression of MIP-1α and IL-2on autoimmune prostatitis in rat model16SD rats were randomly divided into2groups, namely normal control group and modelgroup. EAP model was built by applying purified solution of prostatein supplemented withdiploid immunologic adjuvant. After successful modeling, histomorphological changes ofprostatic tissue, spleen and thymus gland were observed under the light microscopy, with theirviscera coefficients being calculated. Concentrations of IL-2and MIP-1α in the homogenate ofprostatic tissue and serum were tested by ELISA.2. Effect of tacrolimus on the expression of MIP-1α and IL-2in autoimmune prostatitis inrat modelSD rats were randomly divided into8groups: control group1, control group2, model group1, model group2, low-dose tacrolimus for2weeks group, low-dose tacrolimus for4weeksgroup, high-dose for2weeks group and high-dose for4weeks group. After successful modeling,rats in low-dose group and high-dose group underwent gastric perfusion with0.2mg/(kg.d) and0.8mg/(kg.d) tacrolimus respectively for2and4weeks in a successive way. Histomorphologicalchanges of prostatic tissue, spleen and thymus gland were observed under the light microscopy,with their viscera coefficients being calculated. Concentrations of IL-2and MIP-1αin thehomogenate of prostatic tissue and serum were tested by ELISA.Results1.The expression of MIP-1α and IL-2on autoimmune prostatitis in rat modelIn the model group, both glandular cavity wall and interstitium were damaged, edema andhyperplasia of epithelium with cellular infiltration were observed among the interstital tissue andglands. The number of inflammatory cells in normal control group and model group was28.75±18.08,381.25±146.33per square centimeter respectively and the difference wasstatistically significant(P＜0.01).Compared with normal control group, concentrations of MIP-1α and IL-2in the homogenate of prostatic tissue and serum increased in model group (P＜0.05), which showedstatistical significance.2. Effect of tacrolimus on the expression of MIP-1α and IL-2in autoimmune prostatitis inrat modelTacrolimus can reduce the number of inflammatory cells in prostatic tissue. Theinflammatory cells decreased more obviously with the intervention time extension and the doseincrease.Compared with model group, concentrations of IL-2and MIP-1α in the homogenate ofprostatic tissue and serum decreased (P＜0.05), except those of MIP-1α in the serum in low-dosetreatment for4weeks group and high-dose for2weeks group.Conclusion The MIP-1αand IL-2may play an important role in the development of EAPand CP/CPPS may be caused by abnormal autoimmunity. Tacrolimus can reduce the number ofinflammatory cells in prostatic tissue in EAP Rat model. This effect may be related to itssuppression of the production of IL-2and activation of T cells.