Experimental Study Of Diosmin On Treatment Of Chronic Nonbacterial Prostatitis

Chronic prostatitis (CP) is an inflammatory response which is due tostimulating factor of microbial pathogen infection and noninfectious factors,and chronic prostatitis can cause in clinical manifestations such asdiscomfort or pain of prostate area, voiding dysfunction andurethralabnormal secretions. Since its pathogenesis is not very clear and its Clinicalcurative effect is poor, both doctors and patients are not satisfied with eachother. The study found that inflammatory cytokines such as IL-6,IL-8, IL-10played an important role in pathogenesis of chronicnon-bacterial prostatitis. Other studies show that flavonoids can directly acton inflammatory cells, and then inhibit the inflammatory factors such asIL-6, IL-8.DOSM is an natural ingredients of flavonoids compounds, it is rich inedible plants, fruits and vegetables, especially in peel of citrus fruit. It canprotect blood vessels, improve venous tone, improve microcirculation andreduce local inflammation. DOSM is widely used in the treatment ofvenous disease and acute hemorrhoids. Can DOSM be used in thetreatment of chronic prostatitis to have a good curative effect?Two kinds of chronic non-bacterial prostate animal model wereestablished in this study, and then intervention treatment was conductedusing DOSM and cernilton.Differences were observed in tissue pathologyand molecular biology between groups, the mechanism of chronicnon-bacterial pathogenesis and the treatment effect after using DOSM wereto be explored.Pelvic congestion derivation: healthy male SD rats were divided intofour groups randomly: Sham operation(SO), Chronic prostatitis model(CPM), prostat group(PG) and DOSM group，each group had eightrats. SO group were exposed prostatic only and the rest of groupsconducted prostate vein ligation so as to form pelvic congestion, two ratsrandomly selected form each group were confirmed modeling success ornot by HE after thirty-five days. We conduct therapeutic intervention byDOSM and Prostat separately were cordacted after modeling success.Estradiol benzoate-induced remover rat testis method: healthy maleSD rats were divided into six groups randomly: Sham operation (SO),Chronic prostatitis model (CPM), prostat group (PG) and with DOSM high,medium and low dose treatment group, each group had eight rats. Eachgroup were removed rat testis except SO group, then estradiol benzoatewere given by injection after surgery. Two rats randomly selected fromeach group were confirmed modeling success or not by HE after thirty days.We conduct therapeutic intervention by DOSM and Prostat separately wereconducted after modeling success, DOSM has high, medium and low threedoses.The above two rats models were sacrificed after one week ofintervention, taking out prostate for biopsy, to observe pathologicalchanges by light microscopy (HE staining, PAS staining,immunohistochemistry); detect IL-8, interleukin-1β (IL-1β), IL-10,C-reactive protein (CRP), malondialdehyde (MDA), nitric oxide (NO),superoxide dismutase enzymes (SOD), level of tumor necrosis factor α(TNF-α) in rats serum by ELISA; detect the relative gene expression levelsof TNF-α, IL-6, SOD, nitric oxide synthase (NOS2), CXCR2, Ltc4s,prostaglandin E synthase (Ptges2), integrin β2(Itgβ2), integration proteinα-L (Itgal), integrin α-M (Itgam), vascular endothelial adhesion molecule(Vcaml) in rat prostate tissue by RT-PCR. Statistical analyses werereducted using SPSS18.0statistical software.Different levels of prostatic stromal edema loose, vascular dilatationand congestion, lymphocytic infiltration around the blood vessels, a large number of lymphocytes and inflammatory cell such as plasma cellsinfiltrate in the endoplasmic could be observed, lymphocyte infiltrationshowed significantly concentrate to clusters locally, and we can alsoobserve fibroblasts and mast cell were also obvious, and alveolar epithelialcell was significant papillary hyperplasia.The model was successful.In the model of estradiol benzoate-induced remover rat testis method:the expressions of TNF-α, IL-6, SOD, N0S2, CXCR2, Ltc4s, Ptges2, Itgβ2,Itgal, Itgam, Vcaml mRNA in prostate tissue were significantly higher inSO group than CPM group(P＜0.05); the expression of Itgal, Itgam, NOS2,Vcaml mRNA were significanty lower in prostate tissue with Prostattreated than CPM group (P＜0.05); the expression of TNF-α, IL-6, SOD,N0S2, CXCR2, Ltc4s, Ptges2, Itgβ2, Itgal, Itgam, Vcaml mRNA weresignificantly lower in prostate tissue with small doses DOSM treated thanCPM group (P＜0.05). The expressions of IL-8, IL-1β, NO, TNF-α, SODin serum were significant higher in SO group than CPM group in thismodel(P＜0.05); The expression of IL-10in serum was significantly lowercompared with CPM group(P＜0.05); the expressions of TNF-α, IL-8, NOin serum were significantly lower in rats tissue with small doses DOSMtreated than CPM group (P＜0.05), however, MDA, IL-1βin serum had nosignificantly change(P＞0.05); the expression of IL-8, NO in serum weresignificant lower in rats tissue with Prostattreated compared with CPMgroup (P＜0.05), however, the expression of SOD was significantly higherthan in CMP group (P＜0.05).In the model of pelvic congestion derivation: the expression of IL-8,IL-1β, CRP, MDA, NO, TNF-α in serum were significantly higher in CPMgroup than SO group(P<0.05); the expression of IL-8, IL-1β, CRP, NO,TNF-α in blood of rat with DOSM and Prostattreated were significantlylower than the control group(P<0.05), however, the expression of IL-10was significantly higher than the control group(P<0.05). In both of the above two models, the prostate tissue was normal in SOgroup and there was no significant change. There were significantlylymphocytes, plasma cells etc， inflammatory cells infiltration andsignificantly epithelial cell proliferation in CPM group. When theinflammatory cells in prostate endoplasmic of prostate tissue of rats withDOSM Prostat treatment compared with the model group, and epithelialcell proliferation was significantly improved.DOSM as microcirculation regulator had real and significantanti-inflammatory effect, it can reduce inflammation of prostate tissue,improve pathological damage of prostate tissue, have a significantprotective effect on prostate tissue in chronic non-bacterial prostatitis.Therefore, we provide favorable experimental basis for DOSM in-depthstudy on the treatment of chronic prostatitis and the clinical application andpromotion.