Study On The Preparation Of Microbial Fetrilizer Using Oil Shale Semi-coke

At present, with the rapid development of oil shale industry, resulting in a largenumber of oil shale semi-coke, taking up a lot of land resources and damaging thesurrounding ecological environment. As a carrier of the preparation of microbialfertilizer, the oil shale semi-coke could be used fully and reasonable. So that canrealize the resources of oil shale semi-coke, solve the failure problem of oil shale semicoke on the ecological environment. At the same time, Microbial fertilizer prepared inthis way can improve the fertility of soil, promote plant′s growth, increase yields forcrop, reduce environmental pollution and improve the characteristics of soil, and soon, during the process of crop′s growth.First of all, the physical and chemical properties were analyzed to determine thefeasibility of oil shale semi-coke as the carrier in the preparation of microbial fertilizer.The results showed that the main organic matter of oil shale semi-coke is chainalkanes and alkenes, which could be removed at the high temperature of800℃, doesnot produce organic pollution of the soil. After high temperature treatment, themoisture content of oil shale semi-coke could exceed100％within three hours ofsoaking experiment. After dry naturally, its water content decreased to below0.5%byone day. This property is conducive to fix the efficient strains. In addition, the heavymetal ions like Pb, Zn, Cu, Cd of oil shale semi-coke, were determined, its contentsare consistent with the harmless index of composite microbial fertilizer, can be usedas material for the preparation of microbial fertilizer.Secondly, the function or growth of Azotobacter chroococcum, Silicatebacterium and cellulose-decomposing microorganisms in different environment wasstudied. The results of studying of Azotobacter chroococcum showed that its have acertain tolerance with socity, and could growth well in the neutral environment, acidicenvironment has a strong inhibitory effect on its growth. In a certain range, the growthquantity of this nitrogen-fixing bacteria increased with the supply of carbon source. When the element of P was plentiful, additional sources of inorganic phosphorus didnot promote its growth. The mount of fixing-nitrogen of this bacteria could up to3.648mg/L in the culture of pH7and the external carbon source at content of3%.Cellulose degrading bacteria can be a good growth in the amount of NaCl below1%.The optimum pH of the strains in degradation of filter paper is6, and the best nitrogensource and phosphorus source were ammonium sulfate and lecithin. Some of thecarbon source was good for the strain to grow and degrade filter, When the externalcarbon source was lactose, the filter paper degradation rate of this strain increased by20%compared with the culture without external carbon source. In different carbonsource condition, maltose is most favorable to the silicate bacteria, and within acertain range, the ability of dissolving phosphorus and potassium increased with theamount of maltose. The strains in pH5-8could work well, however the acidicenvironment had a strong inhibitory effect on its function. The effect of differentnitrogen sources on the strain was small, while the content of salinity of NaCl was upto1%, the strain still has some capacity of dissolving phosphorus and potassium.Be sure there is no antagonism between the three strains, they were prepared formicrobial fertilizer as fuction strains, and studied the specifications of the productionwithin six months. The results showed that the range of pH was7.46-7.74, waterconcent in19.84%-5.63%of the microbial fertilizer within6months. After180d, thetotal number of valid bacteria microbial fertilizer was35×107cfu/g, each number ofeffective bacteria>107cfu/g, all these were in line with the requirements ofcompound microbial fertilizer product specifications.Then, applied the microbial fertilizers practically, and research its influence onthe growth of alfalfa seedlings and soil fertility. Compared the growth of plant andsoil fertility between matrix-blank and, they were almost the same. This showed thatthe application of oil shale semi-coke has no effect on plant growth and soil fertility.Compared with soil-blank, the application of the microbial fertilizer promote thegrowth of plants, and improve soil fertility. The plant height and root length wasincreased by18.38%and16.46%, and its plant fresh and dry ratio have increased to acertain degree. The available nitrogen, phosphorus and potassium of the soil applied with microbial fertilizer were increased by14.09%,17.58%and15.04%. Butcompared with experiment applied the compound fertilizer and microbial fertilizertogether, the experimental resulet was poor. This is because the fuction of microbialfertilizer be used was related to the organic matter in the soil closely. Which couldprovide nitrogen, phosphorus and potassium, also provides a certain amount of carbonmaterial, this was more advantageous to life activities of the functional bacteria, andincrease the soil fertility, promoting the growth of plant.