Selection Of Pink Tomato Inbred Lines And New Cultivars Resistant To Tylcv

Tomato is an important vegetable because of its high nutrient, high yield, wide utility and popularity among the world, and thus it is widely grown around the world. However in recent years, Tomato yellow leaf curl virus (TYLCV) outbreaks in the world including many provinces in China, which causes huge losses in tomato production. Breeding new varieties of disease-resistant tomato is a fundamental approach to prevent TYLCV.At present, the pink tomato varieties resistant to TYLCV in China are rare and relevant breeding program has just begun. Pink tomatoes taste better than the red tomatoes, and they have a higher nutritional value. Most consumers in China like to eat pink tomato. Breeding new varieties of pink tomato resistant to TYLCV will fit people’s diet habits, reduce vegetable production costs and increase economic income of producers. Therefore, breeding more and better new varieties resistant to TYLCV of pink tomato is of huge importance.In this study, molecular marker-assisted selection (MAS) and traditional breeding is combined to select excellent pink tomato lines resistant to TYLCV. Then they were used as parents to generate hybrids with other elite inbred lines. The research work was carried out throughout three successive seasons for crosses comparison, in order to facilitate breeding of new pink tomato varieties. The main results are as follows:1. There were90lines of high generation breeding material of pink tomato. After field growing and traits investigations for1-3generations for anti-TYLCV by MAS, we screened a number of elite pink tomato lines resistant to TYLCV (including HG2165, F2213-1, etc.), as well as some elite pink tomato breeding lines without TYLCV resistance (e.g. HG9, HG10, HG17and F944-1, etc.), There are also a large number of pink tomato material that are still in the stage of segregation. 2. Cross combination were set up using anti-TYLCV pink tomato lines as male parents and pink tomato with general elite traits as female parents by the top-cross method. The crossing combinations were evaluated in terms of disease resistance, botanical characters, fruit characters, fruit quality index and yield investigation. We have obtained three crosses with elite performance:FH-6, FH-17, FH-19, providing the basis for further breeding work.3. The bacterial wilt (BW)-resistant tomato material is carried out on basis of previous work by Wang Zhen. The inbred lines with BW and TYLCV resistance and high economic traits were utilized as parental lines for crossing combination, giving totally20hybrid combinations. These hybrid combinations were evaluated through2seasons by combination comparison tests. We obtained2hybrid combinations (BW-4and BW-14) with good performance. Especially, the cross BW-4with BW and TYLCV resistance shows infinite growth type, fine fruit shape, high fruit firmness, fruit weight of around210g in Spring season, and medium maturation time, and so its overall performance is the most prominent, and can be used for further regional and production test.