Study On Reproductive Performance And Biological Characteristics Of The Fibroblast Cell Lines In Kunming Dog

As independent cultivation in China only a working Dog breeds,Kunming Doghave been widely promoted and applied in the international and domestic.Accordingto the traditional way of dog breeding produced a good police dog isinefficient.However,animal somatic cell cloning technology can bulk copy good dogs,improve the efficiency of cultivating meritorious police dogs.This researchdemonstrated the basic research on creating the cloning dog.By dissecting the bitches’generative organs,we could see that the ovary was packaged by the bursa ovarica.Thebent and short oviduct was in the bursa ovarica’s parietes.Along with the follicle’sgrowth and development,the ovary’s surface showed a petal structure.Throughout theyear both in Kunming Dog estrus rate is11%,which estrus highest proportion ofSeptember, the proportion of April estrus the lowest was about6%. Bitches eachmonth throughout the year both delivery. The effect of temperature change on thefemale oestrus and delivery is not obvious.Statistics767bitches Kunming Dogs estrusrecords, breeding, reproduction-related records.The breeding rate was89.2%andpregnancy rate was62.6%, the average gestation period was61.6days in Kunmingdogs, bitches average litter number is about7, litter born alive is about6.7.This testselected Kunming Dogs and Belgian Malinois that was1~2years old, each dog inject PMSG500IU/d,a total of five times,then muscular inject HCG500IU on the10thday.Treatment groups oestrus apparent obviously. Effect of oestrus wresignificant.The oestrus rate was100%.While the dogs in control group,other dogs areno oestrus.The B group of Ⅳ treatment group and Ⅴ kunming dogs bitches afterperformance estrus charged with semen artificial sperm.Conception rate of Ⅳ groupis20%(1/5), and conception rate of Ⅴ group is33.33%(1/3).Use of about33days ofKunming dog fetus, the fetal fibroblast cells were isolated and purified by collagenasedigesting and cultured or subcultured in vitro to observe morphology and cell growthsexy identification. The PCR method to identify the sex of dog fetus.The presentstudy could be a reference to help improve the study in cloning Kunming Dog.