Study On The Microsatellite Polymorphism And The Molecular Markers For Cashmere Performance Of Two Cashmere Goat Breeds

Neimenggubai cashmere goat and liaoning cashmere goat's genetic divrsity was studied by 10 microsatellite sites. KAP was selected as candidate gene at the same time, studing its possibility to indirect cashmere yield, weight and cashmere fineness. The result showed: The 10 microsatellite sites was selected and got specific amplified product in every sites,76 allelic were got in all sites and average number was 7.6,the allele number of the 10 microsatellite sites in 2 cashmere goat were between 5 and 11, the effective allele number was the most at BMS3413(N was 6.3411,L was 7.7460), the effective allele number was the least at BMS710(N was 2.7480,L was 2.9438 ), the mean effective allele number of the 10 microsatellite sites in Neimenggubai cashmere goat(N) and Liaoning cashmere goat(L) was 4.4526 and 5.0619 , respectively. The effective number was less than the observed allelic number. This was caused by the inhomogeneity of allelic. The mean site heterozygosity is 0.7601 and 0.7872, respectively. The mean polymorphism information content is 0.7251 and 0.7473 respectively, which indicated that the 2 breeds had abundant genetic diversity. L's heterozygosity and polymorphism information content was higher than N's, indicating that L was good than N in genetic resource. PCR-SSCP's results showed that The mean site heterozygosity is 0.2713 and 0.2872, respectively. The mean polymorphism information content is 0.2251 and 0.2463 respectively. These result were comformed to microsatellite's result, L was good than N in genetic resource. Sequence analysis results showed that there were transition of transversion in 10 places in BB genotype, AA genotype was normal. In cashmere yield, AA genotype was significant difference(P<0.05) with BB genotype at S1 sites, AA genotype was the best marker genotype. AB genotype was significant difference(P<0.05) with BB genotype and AA genotype at S2 sites, AB genotype was the best marker genotype. By calculation selection reaction , S4 site was the best marker site in cashmere yield as L,S2 sites was the best marker sites as N. In weight traits, BB genotype was significant difference(P<0.05) with AA genotype at S2 sites, BB genotype was the best marker genotype, AA genotype was significant difference(P<0.05) with BB genotype at S4 sites, AA genotype was the best marker genotype. Selection reaction showed that S2 sites was the best marker sites as L in weight trait.,S1 sites was the best marker sites as N in weight trait In cashmere fineness trait, BB genotype was significant difference(P<0.05) with AA genotype at S5 sites, BB genotype was the best marker genotype. By calculation selection reaction, S5 sites was the best marker sites as L in cashmere fineness trait.