| In this article, the differences and relationships of polysaccharide and the majorcomponents between the Tricholoma matsutake mycelium and fruitbody were studied. Wehave used orthogonal analysis method to study fruitbody polysaccharides extracted from thebest conditions and single-factor method to study mycelium medium composition and cultureconditions. Have etermined the fruitbody and mycelium polysaccharide composition by themethod of thin layer chromatography test, purified polysaccharides by column chromato-graphy, determined intrinsic viscosity of polysaccharides by the Ubbelohde viscometer.Extracted of effective components, separated and purified of fruitbodies and mycelium toresearch and compare the differences and relationships between them.Finally, the best conditions of the Tricholoma matsutake fruitbody polysaccharide werefollows: extract temperature80℃, extract time1h, added water1:40. The results of singlefactor selection mycelium medium composition and conditions showed that: Tween added0.2%in seed liquid. The best fermentation medium compositions were follows:10%maltose,yeast extract paste3%, corn steep liquor1%; the best fermentation conditions were follows:inoculum size5%, pH5, temperature23℃.Get the relationships of dynamics amongremained carbohydrate, polysaccharide and biomass. Tricholoma matsutake componentanalysis results showed that: thin layer chromatography showed that the composed of fruitbodies and mycelium polysaccharide mainly were same and both of them were constructed byglucose. The results of Sephadex G-200column chromatography separation showed that:fruitbodies and mycelium polysaccharides were mainly composed of two kinds ofmacromolecular polysaccharide. The viscosity of fruitbody polysaccharide was172.40，mycelium polysaccharide viscosity was172.77with Ubbelohde viscometer.Polysaccharides of Tricholoma matsutake fruitbody and mycelia were the same throughthe results of the thin-layer chromatography, column chromatography and viscosity. |
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