Protective Effect Of Tricholoma Matsutake Extract Against UVB-induced Oxidative Stress And Inflammation In Mouse Skin

The skin has been exposed to a variety of environmental pressures,including ultraviolet(UV)radiation.UVB is an inherent component of sunlight that can pass through the human epidermis to the superficial layer of the papillary dermis.UVB induces the production of a large amount of ROS,which leads to oxidative stress,inflammatory response and DNA damage.In recent years,the continuous destruction of stratospheric ozone leads to the continuous increase of UVB radiation on the earth's surface,which poses a major threat to the skin and results in photoaging and skin cancer.Plant extracts and natural compounds have been used to prevent and treat diseases in traditional medicine,but the effects of most of these compounds have yet to be verified.As people pay more and more attention to the quality of life,the cosmetics market of anti-aging and photoprotective products based on natural compounds is growing,and the research in this field is also in high demand.As a precious and rare edible and medicinal fungus,Tricholoma matsutake not only has high nutritional and edible value,but also has a variety of bio-pharmacological effects.In Asian countries,Tricholoma matsutake has been used as a biomedical material to prevent and treat diseases for more than a thousand years,but it is not clear whether Tricholoma matsutake has the effect of anti-UVB-induced skin damage Therefore,it has potential application value and practical significance to study the protective effect and mechanism of Tricholoma matsutake extract(TME)on the skin photodamage.This paper first summarizes the skin structure and function as well as the main mechanism of photoaging.Then,the protective effect and the mechanism of TME on UVB-induced photodamage are described.The main research contents and results are as following?(1)Antioxidant capacity of TME in vitro:The antioxidant activity of TME in vitro was determined by DPPH and ABTS experiments,and their IC50 values were 1.01 mg/mL and 0.41 mg/mL,respectively.(2)Protective effect of TME on HaCaT cells damaged by UVB:The survival rate of HaCaT cells was determined by MTT method.It was found that high concentration of TME could significantly improve the survival rate of HaCaT cells damaged by UVB.(3)Evaluation of the protective effect of TME on UVB-induced skin damage in Kunming mice:The effect of TME on mouse skin was observed by skin histology and pathological sections.The results showed that TME could reduce the skin erythema,wrinkles,edema and epidermis thickening induced by UVB,and also reduce the degradation of collagen fibers,which had a protective effect on the skin damage induced by UVB radiation.(4)Study of the protective mechanism of TME on UVB-induced skin damage in mice:Oxidative stress and inflammation are the main factors causing skin light injury.The results showed that TME could decrease the content of ROS and increase the activities of SOD,CAT,GSH-Px and G6PD.TME had different inhibitory effects on IL-1,IL-6,IL-8 and TNF-?,among which IL-6 and IL-6 were significantly inhibited;TME could reduce the degradation of type I and III collagen,decrease the expression of MMP-3 and MMP-9,and down-regulate the phosphorylation levels of COX-2,p38MAPK and NF-?B.In summary,the results of this study proved that TME has a protective effect on UVB-induced skin damage in mice,and its protective mechanism may be mediated by increasing antioxidant enzymes and down-regulating key cytokines of cellular inflammation.In addition,TME can inhibit p38 MAPK and NF-?B pathway,reduce the expression of MMP-3 and MMP-9,thus reduce the degradation of collagen in mouse skin and ensure the integrity of skin structure and function.Therefore,TME is a potential natural product in skin care and prevention or treatment of oxidative stress and inflammation-related diseases.