The Morphology Study Of The Fetal Vestibular Organ Development Affected By Exercise Training

Development of embryonic Bodily-kinesthetic Intelligence has a profound impact on thenewborn’s intelligence quotient. As one of the earliest developmental sensory system,vestibular system largely determines the Bodily-kinesthetic Intelligence. Vestibulardevelopment is mainly effected by the intrinsic factor of gene regulation and the externalfactor of gravity and exercise, as well as the interaction between the two factors. A lot ofstudies about the gene regulation and the gravity impacting on vestibular development havebeen reported. However, there has been little research on the factor of exercise. Theinvestigation results of human have showed that pregnant women taking part in sports ofswimming, aerobics, yoga, tai chi, gymnastics, and dancing could promote the developmentof vestibular and auditory organs and the growth of the postnatal Bodily-kinestheticIntelligence during middle and late pregnancy. Animal experiment exploring the vestibulardevelopment effected by exercise stimulating for the aspect of structure and function has beenlacked. Our previous research has shown that, the low intensity rotation sports during theperiod of vestibular epithelium mitosis and differentiation (embryo tenth to fifteen day) ofmouse could promote the embryo vestibular function on the birth day. The preliminary resultwas only confirmed that exercise training could promote the vestibular functionaldevelopment, but research on the structure was consistent with previous study or not? Howlong the exercise training time is the most effective? The effect of stimulation of differentsports is difference or not? All of the topics above-mentioned need to study sequentially.In the present study, the Kunming mouse was used. The stimulating form of aroundhorizontal axis rotation and off-vertical axis rotation (OVAR) were choosed. In the period ofE4-E12and E4-E15of embryonic development, the pregnant mice were stimulated with thetow stimulating forms and different duration of rotation per day. In order to detect the degreeof development of the vestibular epithelium, EdU-labeled proliferative cells andHoechst-labeled total quantity cells of vestibular epithelium were counted by microscopy onthe embryonic12.5day (E12.5), and immunohistochemical method or immunofluorescence techniques was used to label the hair cells and afferent fibers in the vestibular epithelium on15.5day (E15.5) and birth day of the newborn. Depending on above experiments, we couldexplore the effect of tow stimulating forms and different duration of exercise on the embryovestibular development in morphology. In addition, the pregnant weight, food intake, times ofactivity and times of stand were detected for revealing the effect of exercise above-mentionedon the pregnant mice.The experimental results are as follows:1.The results that counted the number of cell proliferation (on E12.5) and total quantitycells (on E15.5) in the vestibular epithelium showed that there was no significant differenceamong OVAR group,3h group of around horizontal axis rotation and blank control group(P>0.05). There was no significant difference in the statistical results about the averageweight and average food intake of the pregnant mice among the3groups (P>0.05).2.The statistical results of the number of Hoechst-labeled total quantity cells (on E12.5)in the vestibular epithelium showed that the6h group of around horizontal axis rotation hadsignificant increase compared with3h group and12h group and blank control group（P<0.05）.3.The statistical results of the number of EdU-labeled proliferative cells (on E12.5)showed that the12h group had significant decrease compared with control group（P<0.05）.4.According to the results that counted number of differentiated cells byimmunofluorescence labeled MyosinVII-like cells, there was on significant difference amongblank control group,3h group,6h group and12h group（P>0.05）.5.According to statistical results about pregnant weight, food intake, times of activities,times of stand among all groups of around horizontal axis rotation, the times of activity of12hgroup had significantly decrease compared with other rotation groups（P<0.05）, the times ofactivity of3h group,6h group,12h group had significantly decrease compared with blankcontrol group（P<0.05）, the times of stand of6h group had significantly decrease comparedwith3h group and12h group （P<0.05）.Summary and conclusions: 1.Under the same condition of exercise duration per day, the effect of exercise on thedevelopment of vestibular organ was nothing to do with exercise forms, only related to theexercise duration.2.Rotation stimulation of6h per day could increase the proliferative cells significantly investibular epithelium (on E12.5), and rotation stimulation of12h per day could decrease theproliferative cells in vestibular epithelium (day E12.5).3.Compared with blank control group, all pregnant mice of around horizontal axisrotation groups significantly enhanced the adaptive capacity of environmental changing. Theeffect of6h group was the best. The duration of12h is too long to induce the pregnant miceunhealthy.In conclusion, moderate intensity and low intensity exercise could significantly enhancethe adaptive capacity of environmental changing，however high intensity exercise could harmthe pregnant mice healthy. The moderate intensity exercise could promote the proliferation ofvestibular epithelium cell significantly, and the high intensity exercise could inhibit theproliferation of vestibular epithelium significantly.