| Nanomaterials are particles with diameter between1-100nm. Nano-silica is atypical representative of nanomaterials, and widely used in our daily lives, such ascosmetics, food, watches and electronic. Due to the small size of Nano-silica particle, itcan be floating in the air and intaken by human body through breathing as well as skincontact, having an impact on human health. In addition, Nano-silica can also reachesthe fetus through the blood tire barrier, and is used as abortion drugs in clinical practice.Thus, a certain dose of nano-silica would have a serious harm on the fetus. In recentyears, research on nano-silica showed that nano-silica would have impact on the miceembryo and placenta through the blood of pregnant mice and fetal placental barrier.To illustrate the effect of nano-silica on E15.5day mice placenta, embryomorphology and genes expression levels, Pregnant rats at E15.5day were used as theresearch objects in this study.Nano-silica solution were injected into E6.5-E14.5pregnant mice through intraperitoneal injection, HE staining and quantitative RT-PCRwere carried out to detect the morphology changes of mouse placenta and embryo, aswell as gene expression levels. The effect of nano-silica on mouse embryonicdevelopment were studied, and the results would provide a theoretical basis for the safeuse of nano-silica.Morphological observation and results of statistical analysis showed thatnano-silica can cause E15.5days mouse embryo placental weight and weight reduction:E15.5day mouse placenta into glial cell layer area smaller, E15.5days small mouseembryonic individual smaller. Nano-silica can cause E15.5day mouse embryo placentaand embryonic liver into blood-related genes VEGFa, VEGFc, Flk-1and Ang2gene andmouse embryonic placenta and liver related genes PGF, HGF gene expression generatedchange; nano-silica may inhibit E15.5day mouse embryo testis DNAmethylation-related enzymes Dnmt1expression, while the E15.5day mouse embryotestes and ovaries hormone receptors AR, Esr2genes are not significantly affected.From the above results, the influence of nano-silica to mouse embryo, placenta andgene expression and thus affect the development of mouse embryos. |
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