Effect Of Nano-silica On Mouse Embryos Reproductive System

Nano-silica is a kind of nano-materials, and its application scope is very extensive.It has many excellent properties. Nano-silica has a good application prospect in thepaint, drug delivery and biomedical materials. Mother exposure to nano-silica duringpregnancy can lead to nano-silica through the placental barrier and enter into the fetus,so nano-silica can accumulate in the embryo and affect fetal development. At the sametime, nano-silica can also affect the normal function of the main organs.In order to clarify the nano-silica’s influence on the embryonic development andreproductive systems. Firstly, we will inject pregnant mice abdominal cavity withnano-silica’s, and then take out embryo on E18.5day. We will use qRT-PCR to analyzethe gene expression of liver, brain, placenta and testis and observe pathologic changeof above tissues on the HE stain. Use qRT-PCR to analyze the gene expression of testisand ovaries and observe pathologic change of above tissues on the HE stain when theoffspring of mice grow to four weeks. Judge the change of reproductive capacity onbasis of the quality and quantity of sperm and estrous cycle when the offspring of miceis sexually mature. This research lays the foundation for the nano-silica’s influence onthe embryonic development and reproductive systems and meanwhile it also providethe theoretical basis for nano-silica’s application.Morphological observation and statistics indicates that nano-silica’s will make thefetus lose weight, increase the rate of oaf. HE stain indicates that varying degrees ofpathologic changes of placenta, embryonic liver, embryonic testis, embryonic ovariesand testis of offspring mice and ovaries of offspring mice. qRT-PCR indicates thatexpress quantity of placenta PGF increase, express quantity of Flk1and Flt1decrease,express quantity of placental growth factor peg3increase. VEGFa、Ang2of liver andHGF of liver growth factor increase remarkably mRNA express level of gene ofspermatic Dnmt1,Dnmt3b and Dnmt3l rise remarkably. There is no change on brain ofembryo no matter molecular level and traits level. In forth week, RNA express level ofDnmt1、Dnmt3a、Dnmt3b and Dnmt3l gene lower. Express level of androgen receptorsof testis AR lower. The express of estrogen receptor Esr1has no change. Gene expresslevel of mRNA of Dnmt1,Dnmt3b and Dnmt3l lower remarkably. The express ofovaries of androgen receptor has no change. Nano-silica’s has the negative impact on the reproductive capacity of male mice. The testis has pathologic change, spermmotility will lower, rate of oaf will increase, and fertility capacity will lower.Nano-silica’s also has impact on the reproductive capacity of male mice. Estrous cyclehas no remarkable change. Proestrus will shorten and postestrus lengthen.In conclusion, nano-silica has influence on the development of embryo and hasnegative influence on the reproductive system and reproductive ability of offspringmice.