Establishment And Application Of Post-implantation Mouse Embryo Culture System In Vitro

As a model for studying the early development of mammalian embryos,mice have a history of more than 40 years.It is currently the most extensively and thoroughly studied model animal.Many important events before and after implantation of mammals have been thoroughly studied in mice,and the mechanism of postimplantation embryo development has been the focus of attention of scholars at home and abroad in recent years.The establishment of an in vitro delayed culture system for mouse embryos can enable us to study in depth the relevant mechanisms of mouse embryonic development after implantation,and can also serve as a reference basis for the establishment and optimization of other mammalian embryo culture systems in vitro.However,the generation of EPS cells provides a new idea for the study of mammalian pluripotent stem cells,and provides a unique cell platform for the translational research of biotechnology and regenerative medicine.This paper combines the establishment of an in vitro culture system for mouse embryos to prepare chimeras of EPS cells and early embryos of mice.The development of human and mouse chimeras was studied by in vitro delayed culture technology.CDX2 was identified as co-expressed with EPS cells.Revealed that EPS cells can maintain good cell stemness during the early embryonic development of mice,and t-SNE analysis of chimeric EPS cells by single-cell RNAseq sequencing and biological information analysis technology showed that EPS cells were in The transcriptome level is close to that of normal mouse cells,which is consistent with the results of immunofluorescence.EPS cells are chimerized in the three germ layers of the mouse embryo,revealing the potential application prospects of stem cells in heterogeneous chimerism.