Research On The System Of Tissue Culture And Rapid Propagation For Sorbus Alnifolia

In order to establish efficient system about rapid propagation of tissue culture, In thisexperiment, seed embryos,cotyledons, leaves, petioles, stem segments and buds of Sorbusalnifolia were taken as explants to culture by a series of tissue culture technologies: seeddisinfection, callus induction and differentiation, organ of bud induction and proliferation, strongseedling,rooting and transplanting and so on.In order to filter and determine the most suitablehormone type and concentration ratio for rapid propagation,and obtain healthy and strongplants,to improve efficiency of production. It has practical significance in the rapid propagationof Sorbus alnifolia and cultivation of new varieties.The studied results were as follows:1Seed disinfection: Treating seeds with1%and3%concentration of sodium hypochlorite,in5min,8min, and10min. The result show that the best disinfection method was: disinfectingwith3%sodium hypochlorite for8-10min.2Callus induction: Five kinds of aseptic explants was induced respectively by two hormonecombination. Callus induction by Stem section was significantly better than other explants, thebest hormone combination was MS+6-BA0.5mg/L+NAA0.5-1.0mg/L,the induction rate is100%.3Callus proliferation: Three hormone combination was selected to promote stem callusgrowth, the proliferation multiple of the hormone combination with6-BA+2,4-D is better thanTDZ+2,4-D, then all is better than6-BA+NAA, but the callus in hormone combination with2,4-D+6-BA has a high degree of water damage, the other two hormone combination didn’t existthe phenomenon of water damage nearly. So, the best hormone combination medium wasMS+TDZ0.1mg/L+2,4-D0.1mg/L, the proliferation multiple was4.39.4Callus differentiation:Four hormone combination was selected to differentiate callus. Thehormone combination with TDZ and NAA differentiated more buds, but the bud is translucentand malformationm, and it can’t be used to grow. The callus differentiated in the hormonecombination of6-BA+2,4-D,6-BA+2,4-D+KT was loose and serious phenomenon of waterdamage, it can’t be used to differentiate. The optimum hormone combinations MS+6-BA2mg/L+NAA0.5mg/L,the differentiation rate was up to16%.5Explants of one-step induction of adventitious bud:Petioles,cotyledons,leaves weredifferentiated on the two hormone combination of6-BA+NAA and6-BA+NAA+KT. Thepetioles of bud induction rate was higher than the others, the induction rate was40%, the besthormone combination medium was MS+6-BA5mg/L+NAA0.5mg/L+KT1.0mg/L.6Clump bud generation: Single bud was proliferated in three hormone combination ofTDZ+IBA,6-BA,6-BA+NAA and6-BA+NAA+KT. There is a low multiplication coefficient inthe hormone combination of TDZ+IBA,6-BA+NAA and6-BA, and there is a phenomenon of"cluster bud" in the hormone combination of TDZ+IBA. The best hormone combination medium for clump bud generation is MS+6-BA3.0mg/L+NAA0.1mg/L+KT1.0mg/L, multiplicationcoefficient was23.25.7Rooting culture: MS and1/2MS as the basic medium, add the auxin IBA.The best rootingmedium was1/2MS+IBA0.5-1.0mg/L, rooting rate reached83.3%.