| Camptotheca acuminata Decne of Nyssaceae contains Camptothecin (CPT)-a kind of effective component, which is active to cancer. Salvia Miltiorrhiza Bge of Labiatae is an important traditional Chinese herbal drugs, especially in curing cardiovascular disease. A systematic study on the tissue and cell culture of Camptotheca acuminata Decne and Salvia Miltiorrhiza Bge is significant not only in propagating fine plantlets, but also in producing their active components. With Camptotheca acuminata Decne and Salvia Miltiorrhiza Bge as materials, the experiment established and perfected their tissue culture and plant regeneration systems, determined the active components in them. The main results of this experiment were as follows:1 .A systematic study was made on the induction of callus from terminal bud, cotyledon, plumular axis and the regenerated plantlets from terminal bud in Camptotheca acuminata Decne, and a large number of calli and cluster buds were obtained. The experiment result showed: when the medium was MS supplemented with 2,4-D2.0mg/L, BAl.0mg/L and NAA0.5mg/L, the callus induction rate of the cotyledon was 87.9%, the callus induction rate of the plumular axis was 82.9%, and the callus induction rate of the terminal bud was 6.7%. As the medium was MS supplemented with 2,4-D2.0mg/L, BA1.0mg/L, the callus induction rate of cotyledon and plumular axis were more than 85%, the quality of callus was loose, and the color was light green with a slight red. When the medium was MS supplemented with NAA and BA, the induction rate of callus was lower, and the callus obtained was green and hard. The relatively good medium for bud was MS supplemented with ZT1.0mg/L, NAA0.1mg/L. A better medium for root was MS supplemented with IAA or IBA0.01mg/L and the rate of rooting was 50%.2.The content of Camptothecin (CPT) in callus was analyzed. The result was that different kinds and concentrations of hormones had a great effect on the content of CPT in calli. When the medium was MS supplemented with 2,4-D0.5mg/L, BAl.0mg/L and 2,4-D1.0mg/L, BAl.0mg/L, the CPT accumulated only on a smallquantity. As 2,4-D increased to 2.0mg/L, the CPT accumulated more. The content of CPT in brown calli was higher than that in green calli, and it amounted to 0.827 , close to the original plant young leaves in which the content of CPT was (0.927%o), and the content was far higher than that in the original plant old leaves (0.067 ) and plant covers (0.220 ).3.The induction of callus and regenerated plantlets from stem, leaf and anther were systematically studied in Salvia Miltiorrhiza Bge, and a vast amount of shoots were obtained. The MS medium supplemented with 2,4-D (0.5-2.0) mg/L, BAl.0mg/L was suitable for callus induction and the rate was 100%. The MS medium supplemented with BA(1.0-2.0)mg/L, NAA0.1mg/L was good for the induction and propagation of bud and the induction rate was 100%. The MS medium supplemented with IAA or IBA or NAA0.1mg/L or 1/2MS was suitable for the induction of roots and the induction rate was 100%. The anther could produce adventitious bud on MS medium supplemented with BA1.0mg/L, NAA0.1mg/L.4.Determining the active constituents content of 12 kinds of Salvia Miltiorrhiza Bge that were gathered in Ankang, Shaanxi province arrived at the following: The content of liposolable constituents of Andan No.2 was higher than others. Among them the content of Tanshinone IIA was 0.7085%, the content of Cryptotashinone was 0.1951%, and the content of Tanshinone I was 0.0076%. This laid a solid foundation for the fine seed selection of Salvia Miltiorrhiza Bge cultivars, and for developing and utilizing medical resources of Salvia Miltiorrhiza Bge.5.The research of the effect of different kinds and concentrations of hormones on liposolable constituents content of Salvia Miltiorrhiza Bge calli came to such a conclusion: with leaf , stem, root as explants, the liposolable constituents content of Salvia Miltiorrhiza Bge calli was very low. When the medium was MS supplemented with 2,4-D3.0 mg/L, NAA0.5mg/L, and with the...
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