Tissue Culture And Plant Regeneration Of Ammopiptanthus Nanus (M. Pop.) Cheng F.

Ammopiptanthus nanus (M. Pop.) Cheng f. which was subordinate to Ammopiptanthus Cheng f. of Papilionoideae Taucb in Leguminosae,also called mini- Ammopiptanthus, dwarf- Ammopiptanthus, dwarf- Piptanthus concolor ,is a peculiar evergreen broadleaf bush and carry-over under the third grade in China.Now the plant is under the third-grade priority protection of the state.The results show that A. nanus rely mainly on seed dispersal and found no vegetative propagation,is a type of sexual reproduction. Although the adult plants of A. nanus can tolerate drought in the atmosphere and soil,its seed germination need plenty of water.Due to the long drought windy weather the impact of climate,there are very poor natural environment,perennial drought,about 100 mm of annual rainfall, however, the evaporation rate is greater than 2000 mm in the distribution area,which lead to seed germination is extremely difficult.It was one of the main reasons that of substantial clauses the distribution area reduces gradually.Therefore, develops the A. nanus the vegetative reproduction research has the important meaninf to the A. nanus population expansion. This article uses the tissue culture, the seed and stem section take picks from the Xinjiang Kashi Wuqia county Kangsu town nearby the provincial road 192 kilometer place as the object of study. Main discussing the basic culture medium application, extraneous source hormone use density, the influence test tube seedling takes root main factor and test tube seedling's transplanting, had determined regenerates the way best. Main result like:1 callus cultureThe MS culture medium is the basic culture medium, the hypocotyls of after A. nanus seed sprouts 5d was used to the material,inoculate in the culture medium that add different density 2,4-D, KT,6-BA and NAA,which study the main factor of effect of occurrence of callus.The result indicates when the additional different hormone combination and the hormone density, has the very big difference.And 2,4-D is the key aspect in induceing the hypocotyl callus, the 2,4-D density is when 1.0mg/L the effect is best, The inductivity may achieve above 96%,but KT,6-BA and NAA do not have the tangible effect,2,4-D and the 6-BA combination increases to the biomass has the obvious function,after 60 days the biomass increases above 60 times on MS+2,4-D 1.0 mg/L+6-BA 0.1 mg/L. But does not have to be able to split up grows thickly the bud.2 Seed seedling fast reproduction system's establishmentTake seed seedling of sprouts 20 days as the material.The MS culture medium is the basic culture medium, Has studied the sffect of the different hormone and the different raise condition to the A. nanus clump of bud induction,axial bud induction,elongation growth and the group cultivates the seedling to take root.Result discovery that 1/2MS+IBA 0.2 mg/L+TDZ 0.2 mg/L+NAA 0.5 mg/L +IAA the 0.3 mg/L culture medium and 1/2MS+IBA 0.2 mg/L+6-BA 1.0 mg/L+KT 0.1 mg/L on the culture medium material average clumpy buds is highest,up to five,but they take root with difficulty; The induction axial bud most appropriate culture medium is 1/3MS+IBA 1.0 mg/L, Its axial bud inductivity can 80% above, Will vaccinate takes root on the culture medium later to take root with the growth condition to be good;Takes root the culture medium by 1/3MS+IBA 1.0 mg/L, 1/3MS+IBA the 0.5 mg/L effect to be best, Taking root leads above 75%. As a result of A.nauns to barren environment adaptation, Therefore transplants to the matrix nutrition request is not high, But must maintain the matrix the permeability, The effect which transplants as the matrix is best with the vermiculite, The survival rate is up to 60%.