Study On Light Scattering Information Of Red Blood Cell In The Flow

After200years of development, now light scattering technology has developed aprecision measurement method integrated of optical, electronic technology, computertechnology, signal processing and so on, it widely applyied in cytology, biology,medicine, petrochemical industry, polymer materials and other fields by virtue of itsadvantages: simplicity, fast, nondestructive, accurate and so on. Especially in the fieldof cytology, it not only can determine the size of the cell, cell morphology, but also canget constituents of inside the cell (proteins, DNA, RNA, etc.) by acquiring cellscattering light signal, at the same time, it can identify the asymmetry of the cell and itsorientation in the current system situation, also can understand cell mutations andsurvival situation and to distinguish between them. Red blood cells in the human bodyplays an important role, it becomes a research hotspot in recent years, so this paper withred blood cells as the object of study, established the light scattering research topic ofred blood cell morphology for this article.The paper first elaborated situation of research of light scattering at home andabroad of the red blood cells, and elaborated the basic theory of light scattering and thebasic method of single cell measurement. On this basis, combined with themorphological characteristics of red blood cells, we established the oblate ellipsoid lightscattering model based on the anomalous diffraction approximation, including singleshaft rotary orientation and general situation of three axis orientation. In a single axisorientation, we discussed the influence of light scattering information for differentshape, different size and refractive index and different orientation angle and obtainedthe scattering spectrum distribution through the numerical simulation technology. In thegeneral case of the three axis orientation, we mainly analyzed the influence of thedistribution of the scattering spectrum for different orientation Angle.The paper proved the correctness of the above theory model by using wide anglelaser light scattering instrument BI-200SM, experiment concluded that we can be moreaccurately distinguish between different sizes of red blood cells in the forward smallangle. We confected three different kinds of red blood cell solution: volume ratio1:5000,1:6000and1:8000, measured the dynamic light scattering information andfound through comparative analysis that the scattering intensity is smaller and theeffective diameter is larger in high proportion, the scattering intensity is bigger and effective diameter is smaller in low ratio. Experiment shows that the ratio of differentconcentration will have an impact on measurement results, high proportion relative tolow proportion, it can effectively reduce the complex scattering, which makes themeasuring result more accurate and can be effective for red blood cell sorting, at thesame time, which has important theoretical significance for rapid detection ofpathologic red blood cells, rapid classification, and flow cytometry instrumentimprovement.