Research On Light Scattering Phase Distribution Of Blood Cells Based On VirtualLab Simulation

The exploration of various optical detection technologies on the components and configuration distribution of the biological cells has been an extensively investigated topic over the past few decades since the light scattering method has been applied to the research on biological cells. Many theoretical and experimental results on this topic have been published due to its great effects on life sciences. With the development of digital phase microscopy, phase methods have been effectively used in the research on the detection and classification of cell morphology.This paper is supported by the Natural Science Foundation Project of Jiangsu Province—Optical Detection Method of the Instant Identification of Biological Cells based on the Digital Phase Distribution. At first, the traditional biological cell phase microscopy and the detection methods of biological cells have been fully summarized, and the morphological characteristics and the clinical significance of the various blood cells have been analyzed in detail; Secondly, choosing the typical erythrocyte and leukocyte as the main research object, the paper establishes the hyperboloid rotational symmetrical model of erythrocyte, light scattering phase model of agranular leukocyte, light scattering phase model of granular leukocyte with a cylindrical nucleus and that with a spherical nucleus, based on the real size and morphology characteristics of the blood cells; Thirdly, according to the optical interference theory and related experimental parameters choosing, the VitualLab imitation system is applied to simulate experiments in different incident angles, and extracts the relevant wrapped phase distribution and light intensity distribution by the data analysis model, and then the close and far light intensity distribution is discussed; At last, in order to analyze the phase distribution more effectively, a region grow algorithm led by quality map is applied to unwrap the wrapped phase, and the successive phase distributions are obtained. The phase distributions characteristics of those blood cells are analyzed and discussed through two different angles of different morphologies, and different tropisms in same morphology. In a word, the result shows that the phase information of blood cell is close related to morphology and tropism distribution, so the phase distribution can be applied to exact identification and analysis of cell morphology, and the light scattering phase distribution of biological cell is expected to become a new tool to detect the biological cell. This article is divided into six chapters: Chapter one has introduced the background and the development status of the detection technology of biological cell and the content and the practical value of this project; Chapter two has analyzed the phase microscopy theories and detective methods; Chapter three has established the light scattering phase models of several typical blood cell according to the morphology and optical characteristics of the blood cells; Chapter four has applied the VirtualLab simulation system to experiment, and obtained the light scattering phase distribution; Chapter five has processed the phase distribution, and analyzed the phase characteristics; Chapter six has concluded above researches and presented some other questions which need to be solved in the future.