Type 1 Diabetes Model Oral Glucose Effects On Liver Lipid Metabolism Of Sugar

Insulin is the most effective hypoglycemic drug of diabetes. The complications of diabetes still can’t be completely avoided although blood sugar can be well controled by the continuously improved insulin preparations or pumps. The abnormal lipid metabolism is the most important reason in diabetic angiopathy and complications. Liver, which is a vital organ in glucose and lipid metabolism, and the intersection of exogenous and endogenous lipid metabolism pathways, plays a key role in glucose and lipid metabolism. Therefore, the liver is a suitable research target organ of the glucose and lipid metabolism, and the liver’s glucose and lipid metabolism could be used as an important window to understand the body’s glucose and lipid metabolism.We observed the changes of cholesterol and triglyceride contents in livers of diabetes mellitus mice after oral glucose administration for2hours, and the effects of insulin treatments on liver lipid contents.The results showed that the oral glucose will stimulate the liver cholesterol and triglyceride of diabetes model to increase about1times, and insulin can inhibit the abnormally increase of them. Intraperitoneal delivery of insulin let the diabetic liver cholesterol drop to normal level, and the triglycerides lower than the normal level. Subcutaneous delivery of insulin let the diabetic liver triglycerides drop to lower than normal level, and can decrease the cholesterol level to some extent, but can’t reach the normal control group level.We also analyze the differentially expressed genes of type1diabetic mice’s liver. The results show that, compared with normal control group, the expression of type1diabetes model’s liver lipid synthesis related genes increased2hours after oral glucose administration. The intraperitoneal and subcutaneous administration of insulin can both reduce the expression of type1diabetes model’s liver lipid synthesis related genes.The findings suggest that after type1diabetic mice take oral glucose for2hours, the glucose in liver will convert into lipid abnormally. Insulin can inhibit the abnormal transformation of the glucose and lipid in livers of diabetic mice. Intraperitoneal administration of insulin is more powerful than subcutaneous administration in inhibition of abnormal transformation of glucose and lipid in liver of diabetic mice.The above results not only help to understand the relationship between diabetes glucose and lipid metabolism, and has an important significance for improving insulin treatment strategies, preventing and treating complications of diabetes, but also help to look for new targets of gene therapy and drug development.