| Purposes：Through the high fat diet-induced non-alcoholic fatty liver rat model,observation on liver lipid soluble granule on experimental rat serum HOMA-IR, Leptin andliver tissue TNF-α, PPAR α expression effects on hepatic lipid soluble, particles ofnon-alcoholic fatty liver intervention mechanism.Methods: Will clean in60Wistar rats, to adapt to the environment after7days wererandomly divided into2groups, the normal group9, high fat group51.Normal group weregiven general feeding, high lipid group were fed high fat diet.12weeks later, in the normalgroup selected randomly from1rats, in hyperlipidemia group selected randomly from6rats,were killed after liver tissues were stained with HE.The normal group the remaining ratscontinued to give ordinary feed.High lipid group remaining rats were randomly divided into5groups: control group, model group, hepatic lipid soluble granule high, low dose group,9ratsin each group.Continue the feeding high-fat diet and daily administration of different drugs togastric lavage, normal group and model group were given saline (lml/100g); the control groupwas given the polyene phosphatidylcholine capsule solution intragastrically (0.14g/kg);traditional Chinese medicine group were treated with different doses of liver fat solubleparticles in aqueous solution (3g/kg,2.1g/kg, gastric perfusion0.9g/kg).Consecutive gavagefor4weeks after operation, the liver tissue stained with HE, were observed in the liverpathological change; by radioimmunoassay in detection of serum HOMA-IR, enzyme linkedimmunosorbent assay of Leptin expression, immunohistochemical staining for detection ofliver tissue TNF-α expression and PCR technique for the detection of liver tissue PPAR αexpression.Result：1Liver lipid soluble particle effects on NAFLD rats liver indexThe high dose group of liver index was significantly lower than the control group, therewas significant（P<0.05）; the middle dose group of liver index compared with the controlgroup, no statistical significance（P>0.05）; low dose group of liver index higher than high,middle dose and control group, there was a statistically significant difference（P<0.05）.2Liver fat soluble granule on NAFLD rat liver pathological and morphological effectsThe model of liver tissue steatosis degree is the highest in rats of group, there wassignificant difference compared with the normal group (p<0.01); high, medium, low dosegroup and the control group of rats liver tissue pathological changes were improved, liversteatosis and reduce the number of cytoplasmic lipid droplets in varying degrees, comparedwith with the model group, the difference was statistically significant (p<0.05); high dosegroup rats liver tissue were significantly better than the control group (p<0.05); the middledose group rats liver tissue steatosis compared with the control group, no statisticalsignificance (p>0.05); low dose group, middle dose group compared with control group, therewas statistical significance (p<0.05).3Liver fat soluble granule on NAFLD rat serum biochemical index and HOMA-IRexpression of 3.1Serum biochemical indexesThe model group and drug intervention group rats serum TG, CHOL, FFA were higherthan those in the normal group (P<0.01); high, low dose group and control group were lowerthan those in the model group (P<0.01), there is statistical significance; the high dose groupwas significantly lower than that of the control group (P<0.05), a significant; compared withmiddle dose group and the control group (P>0.05), no significant difference; low dose groupwas higher than that of high, middle dose group and the control group (P<0.05), there wasstatistical significance.Detection results show that the groups of NAFLD rats serum ALT and AST weresignificantly increased in the normal group (P<0.01); Model group rats serum ALT, AST levelthe highest, compared with other drug intervention group (P<0.01), there is significantstatistical significance; High dose group of rats serum ALT, AST level is lower than thecontrol group (P<0.05), there is significant statistical significance; Dose group of rats serumALT, AST level compared with the control group (P>0.05), no statistical significance; Lowdose group of rats serum ALT, AST level significantly higher than the high, middle dosegroup and control group (P<0.01), with statistical significance. Instructions to improveNAFLD rats liver function (ALT, AST), liver lipid soluble particle effect is better than that ofpolyene phosphatidylcholine capsules.Serum free fatty acid FFA in normal group were the lowest, compared with the othergroups (P<0.01), there was significant; the model group was the highest, compared with otherdrug intervention group (P<0.01), there was significant; high dose group, serum FFA wassignificantly lower than that of the control group (P<0.05), there was significant statisticalsignificance the serum content of FFA; the middle dose group compared with the controlgroup (P>0.05), no significant difference of serum FFA content; low dose group were higherthan those of high, middle dose group and control (P<0.01), there was statistical significance.3.2Expression of serum HOMA-IRGroups of NAFLD rats fasting blood glucose, insulin and IR were significantly higherthan that in normal group (P<0.01), there was significant; while the fasting blood glucose,insulin and IR in the rats of model group was significantly higher than that of the highest,drug intervention group (P<0.01), there was significant; high dose rats fasting blood glucose,insulin and IR significantly lower than the control group (P<0.01), there was significant; themiddle dose group rats, fasting blood glucose, insulin and IR lower than that of the controlgroup (P<0.05), there was significant; low dose group rats, fasting blood glucose, insulin andIR compared with the control (P>0.05), no statistical significance.4Liver fat soluble granules on NAFLD rat serum Leptin express impact studiesThe Leptin content in serum of rats in each group of NAFLD were significantly higherthan normal group (P<0.01), there was significant; the serum Leptin in the rats of modelgroup was the highest, compared with other drug intervention group were significantlydifferent (P<0.01), there is statistical significance; the Leptin content in serum of rats in highdose group was lower than that of the control group (P<0.05), there is statistical significance;serum Leptin rats dose content compared with the control group (P>0.05), no statistical significance; the content of serum Leptin in rats of low dose compared with high, middle dosegroup and the control group (P<0.05), there was statistical significance.5Liver fat soluble granules on NAFLD rat liver tissue TNF alpha express impactstudiesThe expression of NAFLD in liver tissue of rats in model group of TNF-α was higherthan that in normal group (P<0.01), with significant statistical significance; expression ofliver tissue in rats of model group was significantly higher than that of TNF-α in the drugintervention group (P<0.01), with significant statistical significance; expression in liver tissueof rats in high dose group of TNF-alpha is lower than that of the control group (P<0.05), withsignificant statistical significance; liver tissue dose group rats TNF-expression compared withthe control group (P>0.05), no significant difference; the expression of liver tissue in rats oflow dose group, TNF-α with high, middle dose group and the control group. Comparison(P<0.05), there was statistical significance.6liver fat soluble granules on NAFLD rat liver tissue PPAR α express impact studiesThe normal group was significantly higher than that in the other groups (P<0.01), therewas significant; the model group was significantly lower than that of other groups (P<0.01),there was significant; high dose group was the most close to the normal group, theintervention group was significantly higher than that of other drugs (P<0.05), there wassignificant; compared with middle dose group and the control group (P>0.05), no statisticalsignificance; low dose group were lower than those in high, middle dose group and thecontrol group (P<0.05), there was significant.Conclusion:1. Liver fat soluble granules can improve experimental NAFLD rat general condition,reduce the intrahepatic lipid deposition, improve the degree of hepatic steatosis.2Liver fat soluble granules have certain protection liver function, reduce thetransaminase, regulating dyslipidemia, improving insulin resistance, restore normal glucolipidmetabolism role.3Liver fat soluble granules obviously decrease serum Leptin expression, reduce the livertissue TNF alpha expression, strengthen liver tissue PPAR α mRNA expression.4Liver fat soluble granules through can reduce intrahepatic lipid deposition, improvehepatic steatosis degree; Protect liver, reduce enzyme, fall fat, and reflect on nonalcoholicfatty liver disease have good control effect.5Liver fat soluble granules by improving IR, inhibition of serum Leptin expression,inhibiting liver tissue TNF alpha expression, strengthen liver tissue PPAR α mRNAexpression, increase insulin sensitivity, regulate lipid metabolism, promote the fatty acidoxidation decomposition, reduce the liver steatosis, thus play on nonalcoholic fatty liverdisease treatment effect, this may be liver fat soluble particle control nonalcoholic fatty liverdisease is one of the mechanisms. |
PDF Full Text Request