Cd14 In Acute Cerebral Hemorrhage, Irak - 4 Irritable The Regulation Effect Of Liver Damage And Relieving Method Of Intervention Study

Objective:To study the influence of Leukocyte differentiation antigen14(CD14) and interlenkin1receptor associated kinase-4(IRAK-4) activity on Hepatic stress injury in acute cerebral hemorrhage.This research will focus on syndrome of phledm-heat and fu-organ excess as a research starting point to observe the changes of liver, colon pathological and the expression of CD14,IRAK-4on lipopolysaccharide signal transduction pathway in acute cerebral hemorrhage. Meanwhile, it would reveal the mechanism of clearing fu-organ and removing phlegm method to regulate the expression of CD14and IRAK-4by the intervention with xinglouchengqi decoction as target. The research would be expected to find out a breakthrough on HIS following acute cerebral hemorrhage characterized by phlegm-heat and fu-organ excess syndrome. It is of great significance in theory and practice as well.Methods:1.The model of acute cerebral hemorrhage was established by injection of collagenase Ⅶ into internal capsule of cerebrum through stereotactic surgery. The rats were randomly divided into blank control group, cerebral hemorrhage group,syndrome of phledm-heat and fu-organ excess model group and xinglouchengqi group. Each group is divided into three time sub-groups (24h,48h,72h) except xinglouchengqi group.xinglouchengqi group is divided into two time sub-groups (24h,48h).10rats in each sub-groups.2.To observe the expression of CD14and IRAK-4in liver tissue and mesenteric lymph nodes with real-time quantitative PCR and HE staining results.3.Each group of data were compared using single factor analysis of variance with SPSS170statistical software,the result is expressed as (x±s).The differences was Statistically significant(P<0.05).Results:Real-time quantitative PCR results of IRAK-4and CD14mRNA in Mesenteric lymph nodes showed that the expression of CD14and IRAK-4mRNA in cerebral hemorrhage group was increased than that blank control group(P<0.05). The results of IRAK-4, CD14mRNA in liver showed that the expression of CD14and IRAK-4mRNA in cerebral hemorrhage group and phledm-heat and fu-organ excess model group were increased than that blank control group(P<0.05). xinglouchengqi decoction could reduce the IRAK-4mRNA expression in liver and the colon,liver pathological injury significantlyConclusion:Gut mucosal barrier is impaired at the early stage of acute cerebral hemorrhage, which may lead to bacterial translocation and intestinal endotoxemia, eventually resulting in hepatic injury. With the hepatic injury getting worse, the Liver is less able to remove endotoxin, leading up to a vicious circle of aggravated endotoxemia. Thus we assume that the positive feedback loop formed between impaired gut mucosal barrier and expression of CD14and IRAK-4is responsible for the exacerbated inflammatory response and the deteriorating to systemic inflammatory response syndrome (SIRS) and/or multiple organ dysfunction syndromes (MODS) at the later stage.The major pathogenesis hemorrhagic stroke is phlegm turbid retention, phledm-heat and fu-organ excess is the main condition for increasing stroke.Therefor, phledm-heat and fu-organ excess is an important factor that affecting the prognosis of cerebral hemorrhage. We speculate that xinglouchengqi decoction could clear the intestinal bacteria and endotoxin, reduce the LPS, CD14and IRAK-4expression, suppressor NF-κB activity by the intervention of acute cerebral hemorrhage liver injury with xinglouchengqi decoction. xinglouchengqi decoction blocking effection on biological effects of Intestine-liver axis disorders in cerebral hemorrhage period,and thus against intestinal endotoxemia in acute cerebral hemorrhage liver injury and protect the liver.The research provide experimental evidence for the study of acute cerebral hemorrhage by Chinese medicine treatment.