| ObjectivesThe abnormal expressions of ERCC1, EGFR, Ki67and P53genes have been shown to be implicated in the occurrence and development in many kinds of human cancers including some esophageal cancers. However, the research about the relation between the co-detection of the four proteins and esophageal cancer is less and controversial. Up to now, no specific biomarker has been reported to be sensitive and specific for esophageal cancer detection. But, we could improve the detection rate of tumor by comprehensive analysis of a variety of cancer gene and complementary effect. The co-detecting of theses multi-cancer genes expression profiles, therefore, might be helpful for the diagnosis and prognostic evaluation for esophageal cancer.In the present study, ERCC1, EGFR, Ki67and P53genes expressions have been co-detected and their associations with the clinicopathological characteristics of the disease have been investigated with the aim to elucidate the roles of these genes in esophageal cancer occurrence and development and their evaluation in the cancer detection and prognostic assessment.Materials and methods 61patients of esophageal cancer tissue samples and their corresponding tumor-adjacent normal tissues were gathered from the Department of Pathology of the Second Affiliated Hospital of Zhengzhou University between2009and May2012. Material includes the patient’s age, gender, general classification, infiltration depth, tumor location, clinical stage, tumor size and have without lymph node metastasis, etc. The expressions of ERCC1, EGFR, Ki67and P53proteins were detected by immunohistochemical staining in formlin-fixed paraffin embedded samples. Datas were analyzed by SPSS17.0software pack. A Chi-square test with a Fisher extract test and the Spearman correlation was used in this test. P<0.05was considered as statistical significance.Results1. Positive rate of ERCC1, EGFR, Ki67and P53expressions in esophageal cancer were31.2%(19/61),77.1%(47/61),60.7%(37/61) and62.3%(38/61), respectively; and in the corresponding tumor-adjacent normal tissues were49.2%(30/61),9.8%(6/61),16.4%(10/61) and4.9%(3/61), respectively. EGFR, Ki67and P53expressions in cancer were much higher than in the corresponding tumor-adjacent normal tissues (p<0.05), while the ERCC1expression in normal tissue was lower significantly than in esophageal cancer tissue (p<0.05).2. ERCC1expression in esophageal cancer had obvious correlation with degree of differentiation, tumor size and lymph node metastasis (P<0.05). It had no correlation with age, gender, tumor location, general classification, infiltration depth, clinical stage (P>0.05).3. The expression of EGFR had obvious correlation with differentiation and lymph node metastasis (P<0.05). It had no correlation with the age, gender, tumor size, tumor location, general classification, infiltration depth, clinical stage (P>0.05).4. Ki67expression had obvious correlation with tumor location, degree of differentiation, lymph node metastasis, invasion depth (P<0.05). It had no correlation with the age, gender, tumor size, general classification, clinical stage (P>0.05).5. The expression of P53had obvious correlation with gender, degree of differentiation, infiltration depth and lymph node metastasis (P<0.05). It had no correlation with the age, tumor size, tumor location, general classification and clinical stage (P>0.05).6. The protein expression of Ki67had positive correlation with P53in esophageal cancer tissue (r=0.274, P=0.033), but the rest had no obvious correlation.ConclusionsThe abnormal expressions of ERCC1, EGFR, Ki67and P53genes have close relationship with the occurrence and development of esophageal cancer, and the co-detection of these proteins might be helpful for the cancer detection and prognostic evaluation. Ki67protein expression has positive correlation with P53expression in esophageal cancer tissue, indicating that they may have prompting effects with each other, but the specific mechanism of the action remains to be further studied. |
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