The Study Of Occupational Exposure Dose Assessment Method Of Pesticides With Allura Red As Surrogate

The occupational exposure assessment of pesticide plays a very important role in pesticidemanagement, to protect the health of applicator and environment. However, the study ofoccupational exposure assessment of pesticide has just started in China， so set up an accurate andfast exposure assessment method which adapt to national conditions is a problem to be solved.This study used the whole body method to assess the exposure dose for applicator, and to exploreoccupational exposure dose assessment method of pesticides with allura red as surrogate.Developed the examination methods of pesticide chlorpyrifos and dye tracer allura red, andapplied these methods to occupational assessment of pesticide in field, collected the exposure dateof60cm and120cm corn,60cm cotton, analyzed the exposure distribution of two compounds.Pesticide impurities is an important index of pesticide quality and safety performance, the pesticideregistration departments over the world have corresponding limits. Developed a determinationmethod of imidacloprid and applied to the exposure assessment of40cm peanut, and found a mainimpurity in imidacloprid technical. In this paper, the experimental results are as follows：The detection methods to chlorpyrifos and dye tracer allura red have been established andvalidated. The exposure dose of applicator to chlorpyrifos was detected by GC-FPD. LOD andLOQ are0.005and0.017μg/mL, respectively. The recoveries of coverall, gloves and XAD-2arebetween88.3-100.0%, RSD is bellow1.29%. The exposure dose of applicator to allura red wasdetected by HPLC. LOD and LOQ are0.0017and0.005μg/mL, respectively. The recoveries ofcoverall, gloves and XAD-2are between87.8-103.9%, RSD is bellow0.6%. Both of themethods are satisfied the requirement of analysis.Studies of occupational exposure assessment of pesticide, the similar procedure was donewith allura red as a surrogate. An applicator spraying chlorpyrifos and allura red to60cm corn and60cm cotton in greenhouse,60cm corn in field. The main exposed part is the lower part to allexperiments, to three sets of allura red, the UE is between402.70-957.02μg/g, taking up73.58-98.82%of the total UE of applicators; to the two sets of chlorpyrifos, the UE is between123.23-409.36μg/g, taking up90.40-98.37%of the total UE of applicators. However, to the120cm cornin greenhouse, the important contaminate part is still the lower part of body, the UE of allura red sets are among733.31-2375.54μg/g, taking up65.71-84.83%of the total UE of applicators; theUE of chlorpyrifos sets are among424.85-547.45μg/g, taking up53.69-58.29%of the total UEof applicators. The height of crops, the habit of applicator and the spraying device all affect theresults; to the upper part of body, all of experiments taking up less than1%except one chest ofone test is2.2%. The exposure dose of inhalation are very little, the UE are among0.02-1.66μg/g, taking up less than0.51%of total exposure. The detection method to imidacloprid has beendeveloped and validated, applied to the exposure assessment of40cm peanut. A main impurity-BPMNA was found in imidacloprid technical, it was synthesized and the analysis method of it wasestablished, can be directly used in the formulation of imidacloprid technical and the impuritycontent detection.There are a lot of work to do for further study in pesticide occupational health risk assessment inour country, to ensure the scientific management and application of pesticides, to provide reliable basisto protect human health.