| Objectives: Colorectal cancer is one of the most common malignanttumors.The cancer suppressor genes inactivation and cancer genes activationinvolve in the occurrence and development process of the colorectal cancer.NGX6gene，who is one of cancer suppressor genes may delays the tumorprocess by inhibiting the cell proliferation and reducing the expression of cellcycle protein and inhibiting the formation of blood. TUSC4gene who is alsoone of cancer suppressor genes，it may has followed biological function:involving in DNA mismatch modified and regulating of cell cycle signaltransduction and inducting apoptosis. The effect of TUSC4on the colorectalcancer has been seldom reported at home and abroad, investigation oncombined test for the influence of NGX6, TUSC4in colorectal cancer andadenomas has not been reported before.Therefore，we researched the expression and distribution of NGX6andTUSC4in colorectal cancer and adenomas, analysised the relationshipsbetween the clinical pathological data and their correlation，for further discussthe relationship between NGX6, TUSC4and the occurrence and developmentand prognosis of colon cancer， and to provide theoretical basis foroccurrence，development and prognosis of colorectal cancer.Methods:Step one: selecting surgical excision specimens of colorectal cancer for60cases，in Sichuan Provincial People’s Hospital，duringx~2009.01-2012.01.Step two: selecting50cases with surgical excision specimens of colorectal adenomas，include11specimens of tubular adenomas,11specimensof tubulovillous adenomas,11specimens of villous adenomas and17specimens of serrated adenomas.Step three:selecting30cases with surgical margin beyond5cm tocolorectal cancer for controls.EnVision-two-step of immunohistochemistry is used to test theexpressions of NGX6, TUSC4in colorectal cance，colorectal adenomas andnormal tissue, to analysise their relationships and correlation with the clinicalpathological data.Statistical Methods: The analysis of date is performed by SPSS16.0. AsP<0.05is considered statically significant and P<0.01is considered obviouslydifference. x~2test is used to compare the expressions rate; the Spearman testis used for testing the correlation of expressions.Results:1. The positive expression rate of NGX6in colorectal cancer，adenomasand normal tissue is46.7%,84.0%,93.4%respectively, the difference isobviously significant (x~2=28.000P<0.01); the expression of NGX6incolorectal cancer is lower than that in adenomas, the difference is obviouslysignificant (x~2=16.427P<0.01); the expression of NGX6in colorectal canceris lower than that in normal tissue, the difference is obviously significant(x~2=18.529P<0.01); but the expression of NGX6is with no significantdifference between adenomas and normal tissue (x~2=1.493P>0.05).2. The expression of NGX6is closely related with the depth of theinfiltration and lymph node metastasis. The positive expression rate is34.1%and81.2%respectively in infiltration serous layer and no infiltration serouslayer, the difference among them is obviously significant (x~2=10.484P<0.01). Meanwhile, the positive expression rate is19.2%and67.6%respectively inlymph node metastasis group and no lymph node metastasis group, thedifference among them is obviously significant (x~2=13.876P<0.01). Theexpression of NGX6in colorectal cancer is not closed to patients’ age, sexand the tumor size, location and differentiation, TNM stage and so on(P>0.05).3. The positive expression rate of TUSC4in colorectal cancer, adenomasand normal tissue is48.3%,90.0%,93.4%respectively, and the difference isobviously significant (x~2=32.038P<0.01).Among them,the expression ofTUSC4in colorectal cancer is lower than that in adenomas, the difference isobviously significant(x~2=21.506P<0.01);The expression of TUSC4incolorectal cancer is lower than that in normal tissue, the difference isobviously significant (x~2=17.440P<0.01);while the expression of TUSC4iswith no significant difference(x~2=0.261P>0.05)between adenomas andnormal tissue.4. The expression of TUSC4is related with tumor differentiation, TNMstage, lymph node metastasis, the depth of the infiltration. The positiveexpression rate of TUSC4is40.4%and76.9%respectively in low andmoderate differentiated group and high differentiated group, the difference issignificant (x~2=5.432P<0.05); the positive expression rate of TUSC4in TNMstage III+IV group is lower than in I+II group(15.4%,73.5%), the difference isobviously significant (x~2=19.946P<0.01); as well as lymph node metastasisgroup and no metastasis group(19.2%,70.6%), the difference among them isobviously significant (x~2=15.561P<0.01);the positive expression rate is38.6%and75.0%respectively in infiltration serous layer and no infiltrationserous layer, the difference among them is significant (x~2=6.213P<0.05); The expression of TUSC4in colorectal cancer is not closed to patients ’age, sex,location and the tumor size (P>0.05).5. The expression of NGX6is significantly correlated with theexpression rate of TUSC4, and the correlation coefficient is0.573(x~2=x~29.377P<0.05).6. The positive expression rate of NGX6in serrated adenomas, tubularadenomas, tubulovillous adenomas and villous adenomas are82.3%,81.8%,90.9%,81.8%respectively, the difference among them is not significant(x~2=0.503P>0.05).7. The positive expression rate of TUSC4in serrated adenomas,tubular adenomas, tubulovillous adenomas and villous adenomas are88.2%,90.9%,90.9%,90.9%respectively, the difference among them is notsignificant (x~2=0.089P>0.05).Conclusions:1. The expression of NGX6and TUSC4is high in normal tissue andadenomas respectively, but the expression of them is observably reduced incolorectal cancer, suggesting that NGX6and TUSC4by lower expression ordeletion involve in the process of adenomas canceration.They play importantroles in the occurrence process and involve in development process ofcolorectal cancer.2. The expression of NGX6is closely related to the depth of theinfiltration and lymph node metastasis of colorectal tumour.The deeper ofinvasion, as well as lymph node metastasis, NGX6has the lower expressionthat suggesting NGX6may be associated with the prognosis in colorectalcancer.3. The expression of TUSC4is related to differentiation of colorectal tumour, TNM stage, lymph node metastasis, the depth of the infiltration.Thelower of differentiation and the later of TNM stage, as well as lymph nodemetastasis, the deeper of invasion, TUSC4has the lower expression thatsuggesting TUSC4may play an important inbibitional role in the occurrenceand development and prognosis process of colorectal cancer.4. The expression rate of NGX6and TUSC4in colorectal cancer iscorrelated with each other. Combined test NGX6and TUSC4in colorectalcancer may provide new molecular markers to occurrence，development andassessment the prognosis.5. NGX6and TUSC4are expressed no difference in different types ofadenomas, that maybe related to their own nature, but do not exclude thepossibility for the lack of enough sample sizes, so we need to expand thesample sizes for further research. |
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