The Expression Of DCLK1 In Colorectal Cancer、Colorectal Adenomas And The Clinical Research

Backgroundcolorectal cancer (colorectal cancer, CRC) is a common digestive system cancer, the incidence rate is increasing year by year in the world. Doublecortin like kinase-1, (Doublecortin-like kinase 1, DCLK1) is a member of the protein kinase superfamily and doublecortin family, which encodes a gene located on human chromosome 13q13. Recent studies showed that DCLK1 had cancer stem-like cells features in colorectal cancer cell lines and associated with tumor metastasis and poor prognosis. Currently there were few reports on DCLK1 in adults with colorectal cancer and colorectal adenoma tissue contrast.The relationship between its expression and diabetes were also rarely reported.ObjectiveTo detect the expression of DCLK1 in colorectal cancer, colorectal adenoma tissue, analysising the role of DCLK1 playing in the process of colorectal cancer,as well,exploring the DCLK1 expression and prognosis of patients which with diabetes medical history.MethodsThe expression level of DCLK1 protein in 70 cases of colorectal carcinoma,70 cases of normal tissue adjacent to carcinoma and 35 cases of tubulovillous adenoma were detected by immunohistochemistry (SP method).Analyzing its relationship with clinical pathological grade, depth of invasion, lymph node metastasis and other clinicopathological features. Explore history of diabetes correlated with the DCLK1 expression, followed up with a history of diabetes in patients with colon cancer 5-years survival prognosis.Results1.The DCLK1 expression rate in colorectal cancer was 41.4%(29/70), significantly higher than the normal colon tissue adjacent to carcinoma 4.3%(3/70) (P< 0.001), and in tubular villous adenoma,the high expression rate was 45.7%(16/35).The difference was statistically significant (P<0.05).2. There was no significant correlation among DCLKl expression and patients’ sex, age, tumor size in colon cancer (P> 0.05), histological grade Ⅱ level DCLK1 high expression rate was 19%, in stage III high expression rate of 51%, the difference was statistically significant (P <0.01); invasion to the submucosal and mucosal storey expression was 12.9%,55.6% infiltration into muscle, and serosa serous infiltration of 66.7%, among the three groups the difference was statistically significant (P<0.05); expression without lymph node metastasis was 26.5%, with lymph node metastasis of 55.6%, the two groups was statistically significant (P<0.05); no vascular invasion was highly expressed 19.1% compared with 87% having vascular invasion, the difference was statistically significant (P<0.05); no distant metastasis was 26.4% expression, distant metastasis reached 88.2%, the two groups have statistically significant (P<0.05).3.The history of diabetes patients with intestinal polyps and colon cancer history, tumor size, metastasis, high and low, the survival time of patients carcinoembryonic antigen had no significant correlation (P> 0.05), but with a history of hypertension, age had statistically significance (P<0.05). History of diabetes and colon age, history of intestinal polyps adenoma patients had no significant correlation (P> 0.05), with sex was statistically significant (P<0.05), male incidence of colon adenoma with diabetes 41.2%,83.3% of women, thus women had higher risk of colorectal adenomas than men with diabetes. No significant difference between the DCLK1 expression and diabetes in colorectal cancer and colorectal adenomas.Conclusions1.There were abnormally high expression of DCLK1 in colorectal cancer, colorectal adenomas, and the expression in precancerous lesions of the colon adenoma were greater than colorectal cancer, which prompted the important role of DCLK1 occurred in colorectal cancer.Therefore,DCLK1 may be used as an early diagnosis indicator.2.There were no significant correlation between DCLK1 expression and patients’sex, age, tumor size in colon cancer (P> 0.05), but compared the DCLK1 expression among pathological grading, depth of invasion, lymph node metastasis, vascular invasion, distant metastases, there were statistics significance (P<0.05).3. The DCLK1 expression and prognosis in colorectal cancer,colorectal adenomas had no obvious correlation with diabetes medical history.