The Impact Of Different Exercise Intensities On GLUT4Content In Type2Diabetic Rats Quadriceps Femoris

Type2diabetes,also known as non-insulin-dependent diabetes mellitus is ametabolic disease caused by the interaction of many factors, accounting for90%ofthe total number of diabetic patients around the world. Among them, insulinresistance of type2diabetes is an important physiological basis. Skeletal muscle is thebody of the main glucose intake and organizations, glucose transmembrane movementis the first step of skeletal muscle use glucose, in this process, glucose transporter4(glucose transporter4, GLUT4) plays a very important role.Studies have shown thatGLUT4abnormal expression is the important factors that lead to insulin resistance,exercise can reduce blood glucose, insulin resistance drops, improve the content of theskeletal muscle GLLUT4, and thus play the role of prevention and treatment ofdiabetes.Objective: To investigate the effect of disposable different intensity exercise on bloodglucose, plasma insulin levels and the GLUT4content in high-fat feeding rat skeletalmuscle.Methods: In this paper,35male SD rats for the study, randomly selected5as thecontrol group (group C), with the basic feed normal feed. The rest of the30for fourweeks high fat feed, establish type2diabetes model. After the success of the model tobe built of diabetic rats20only randomly divided into four groups, namely thenon-exercise group diabetes (E0group),diabetes exercise1group (E1group), diabetesexercise2groups (E2group), diabetes exercise3groups (E3group), each group of10rats. The exercise group of diabetes rats with different strength on the one-timemovement, namely E1run speed for10m/min (about30%VO2max, little strength),E2for15m/min (about50%VO2max, moderate intensity), E3for20m/min (about70%VO2max, big strength), sports time for40minutes. One-time movement trainingended, with sodium pentobarbital anesthesia abdominal aortic blood take5ml, and letrest for30min,3000r/min centrifugal5min, separate serum, at-70°C refrigeratorfor testing. Take rats after quadriceps, immediately with tin foil package, label, andthen put into the container with liquid nitrogen container, put them-70℃in therefrigerator for later use. Immediately after taking rat quadriceps, with aluminum foil,wrap, label, and then put them in container of liquid nitrogen, and then into the-70°C refrigerator. Testing indicators: fasting blood glucose (FBG) using blood glucosemeter test; serum insulin (FINS) using the ELISA test.Western blot detection ofskeletal muscle GLUT4content.Results:(1)Before a motion, the control group (group C) rats blood glucose was significantlylower than the diabetes each group (E0group, E1group, E2group, E3group) rats bloodglucose. Among them, the blood glucose of diabetic non-exercise group and diabeticexercise group before exercise have no significantly difference. After an exercise,E1group, E2group, E3group rats blood glucose obviously decreased. The E2group wasmost obvious.(2)After one-time movement,the insulin levels of diabetic exercise group haveincreased.The E2group increased the most obviously. The IRI of the three diabetesexercise groups decrease significantly.(3) After exercise, diabetic exercise group (E1group, E2group, E3group) rats theGLUT4relative content were increased. Among them, the GLUT4content of E2group rats increased the most obvious.