| Objective: The glucose metabolism of nerve cells plays an important role in maintaining the function of the nervous system, and the glucose transport is the key link in the whole process of glucose metabolism. In the glucose transport protein family(GLUTs), only GLUT4 can serve as glucose transporter in the body under stress, and provide the energy needed by the body under stress. The skeletal and heart muscle cells,especially skeletal muscle cells due to exercise-induced skeletal muscle contraction on glucose stress demand, which is responsible for the mechanism of glucose supply is mediated by GLUT4 and a number of studies found that Ca2+-Ca MK signaling pathway to the regulation of skeletal muscle and cardiac muscle GLUT4 expression. The sharp thinking activity is similar to the motion, also need to consume large amounts of glucose,nerve cells of a stressful events. Therefore, we can infer that the possible uses a similar to skeletal muscle cells and myocardial cells of the same mechanism responsible for glucose transport.This study through the design of an exercise stress test to explore exercise stress whether can also cause the high expression of rat hippocampus,cerebellum GLUT4 specificity, and motion from a single analog signal-- "caffeine induced, the specific activation of intracellular Ca2+ signal" experimental of study the mechanism of regulation and control. In order to provide theoretical basis for the improvement of the function of the nerve cell glucose metabolism.Methods: Through the two part of the study designed to investigate whether exercise stress can induce the GLUT4 specific high expression in the hippocampus and cerebellum of the rat, and study its regulation mechanism from the Ca2+ signal of the cell. The study one(second parts), in order to explore whether exercise stress can cause GLUT4 specific expression in the hippocampus and cerebellum of rats. Adult male SD rats were 32. By Morris water maze eliminate congenital ability of learning and memory and 16 rats, the remaining 16 rats(followed two groups of escape latency time of the difference between means minimum, independent samples t test p value is the bigger the better) divided into training group(SE) and a sedentary group(SC). The SE group took regular 5 weeks of moderate intensity swimming training, while the SC group had no intervention for 5 weeks without any intervention. After the end of the training, by Morris water maze test se group and SC group learning and memory ability, disposable strength exhausted swimming(weight 7%) measured athletic performance, and the Western blotting detection of biological technology to detect rat hippocampus,cerebellum total cellular protein GLUT4 protein expression levels. The study two(the third part), We study the regulation mechanism of the signal from a single movement, "Caffeine induced specific activation of Ca2+ signal" experiment(The advantage: the effect of the high expression of GLUT4 protein induced by exercise induced AMPK signaling pathway was ruled out).24 adult male SD rats were divided into 4 groups according to their body weight. 2H injection of Caffeine group(C2) and 2H injection of DMSO group(D2) after the completion of the injection of 2H materials, through the Western-blotting biological detection technology to detect the level of Ca MKII(Thr286)in the hippocampus and cerebellum. 24 h injection of Caffeine group(C24) and 24 h injection of DMSO group(D24) after the completion of the injection of 26 h materials,to detect the total protein GLUT4 protein levels in the hippocampus and cerebellum.Results: Study one:(1) the total protein GLUT4 protein of hippocampal cells in group SC was 12.42 times(P=0.009<0.01) in group SE;(2) the total protein GLUT4 protein of SC group was 1.67 times(P=0.014<0.05) in the SE group;(3) the SE group exercise performance was 2.54 times(P=0.000<0.01) of the SC group;(4) SE group of 4 days to escape the incubation period of time compared with the SC group(P=0.3>0.05), no significance.The results showed that the levels of total protein GLUT4 in the hippocampus and cerebellum of SC group were significantly higher than that in the SE group, the exercise performance of SC group was significantly higher than that in the SE group, and there was statistical significance. Study two:(1) the total protein GLUT4 protein of hippocampal cells in C24 group was 1.277 times that of D24 group(P=0.008<0.01);(2) the total protein GLUT4 protein of C24 group was 1.4 times(P=0.047<0.05) in the D24 group;(3) the phosphorylation level of C2(Thr286) in Ca MKII group was 1.491 times that of D2 group(P=0.002<0.01);(4) the phosphorylation level of Ca MKII(Thr286) in C2 group was 7.735 times(P=0.006<0.01)in D2 group. The results show that the phosphorylation of C24 rats hippocampus,cerebellum total cellular protein GLUT4 protein levels and C2 group rat hippocampal and cerebellar cells Ca MKII(thr286) are significantly higher than the control group D24 and D2 and were statistically significant.Conclusion:(1) Relative to take 5 weeks of regular moderate intensity swimming training exercise adaptation group, disposable strength exhausted swimming(weight%)stimulation can cause without any training rats hippocampus and cerebellum of GLUT4 protein is strongly expressed. It showed that GLUT4 had the same effect on the glucose transport in the hippocampus and cerebellum of rats during stressful events.(2) Through the design of cellular Ca2+ signal specific activation agent caffeine induced experiments to simulate an exercise stress test detected C2 group rats hippocampus, cerebellum cell calcium / calmodulin dependent protein kinase II(Ca MKII) phosphorylation and C24 rats hippocampus, cerebellum GLUT4 protein expression levels were significantly higher than those in the control group D2 and D24. It is indicated that the "Caffeine-Ca2+-Ca MKII" signal pathway is present in the regulation of GLUT4 protein expression in the hippocampus and cerebellum of the rat, which is induced by the "Caffeine" signal. |
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