| Type2diabetes mellitus (Type2diabetes) the earliest known as non insulin dependentdiabetes mellitus, because the human body into the middle and old age, the body of thefunction decreased significantly, CO stimulation induced body coupled with a variety offactors outside of pathological changes, according to the latest survey results show, diabeticpatients, the number of patients suffering from type2diabetes is the biggest proportion, about9times as much as other types of diabetes. Pathogenesis of type2diabetes, a large part of thereason is because the insulin resistance induced by. The human body needs to consume energyin motion, and sugar as a function material plays a very important role in the body duringexercise, skeletal muscle can uptake and utilization of glucose in the body when in motion,because glucose to the particulate matter in the body, so the body can not by active transportways out of the cell, it needs some auxiliary transport carrier, in the cell membrane of skeletalmuscle is attached to a glucose transport carrier, the carrier protein transport of glucose iswhat we call the glucose transporter-4(GLUT4), from which we can know, GLUT4translocation in skeletal muscle glucose plays a vital the role of experimental research formany years, according to the analysis results, the incidence of diabetes is due to the body’sinternal caused by insulin resistance, glucose transporter-4(GLUT4) expression abnormalityis the most direct reason induced insulin resistance. The experimental findings, exercise canexert a great influence on the content of GLUT4, the body in motion, will find the contentattached to the skeletal muscle cell membrane on the GLLUT4have increased significantly,the increase of GLUT4content will improve the transport capacity of the body to glucose, soto reduce insulin resistance has the obvious effect, but also indirectly increase the body’sabsorption of glucose utilization, and ultimately to reduce the blood sugar level, blood glucosehomeostasis in patients with the purpose to achieve, so you can control the blood glucoselevels of diabetic patients is effective, and it will be controlled in normal level, this to acertain role in the prevention and treatment on.Research purposes: by applying a month feeding high sugar and high fat diet to SD rats, toobserve the disposable moderate intensity, different time exercise on glucose transport in ratskeletal muscle protein-4(GLUT4) and plasma glucose insulin production.Research methods: This paper from a random sample of60healthy male SD rats in10, with amonth based on feeding feed these rats, and then select one of the8rats as normal controlgroup (group C), standby as normal group of2rats left, high sugar, high fat diet feed theremaining50rats for a month, to prepare the model of type2diabetes, diabetic rats from50randomly selected40rats, were randomly divided into5groups,8rats in each group,10 rabbits as experimental group reserve surplus, then classified to5groups: experimental groupdoes not participate in the motion (group V), continuous movement of20min group (groupT1), continuous movement of40min group (group T2), continuous movement of60mingroup (group T3) and moderate intensity exercise80min (group T4). Applied in theexperimental group exercise stimulates the one-time moderate intensity (50%VO2max,15m/min) of the motion stimulus, at the end of exercise rats after24h, rest, the body, the ratswere anesthetized with pentobarbital sodium injection, and then use the syringe acquisition of5ml rat peritoneal blood, standing for30min, the acquisition of the abdominal cavity arterialblood on the speed centrifuge for3000r/min by centrifugal separation after5min, the serumis placed in the refrigerator to save-70℃, tested. Wrapped with aluminum foil extraction ofrat femoral head four muscle tissue, and marked, immediately into the prepared cake filledwith liquid nitrogen vessel, the extraction of rat tissue temperature refrigerator in-70℃incryopreservation, stand-by. Measurement: to detect the content of GLUT4in skeletal muscleby Western Blot blot; ELISA test method to measure the serum insulin (FI) content in thebody; blood glucose testing fasting blood glucose meter (FBG) at tokyo.The experimental results:(1) a motion before the rats blood glucose were detected out, normal control group (group C)glucose content was relatively low, and diabetes mellitus group (V group, T1group, T2group,T3group, T4group) glucose content in rats is significantly higher than the normal controlgroup. Compared to the pre exercise, blood glucose levels of rats in each experimental groupwere decreased to different extent, the decline in blood glucose levels of rats in the T3groupof the most obvious.(2) compared with the level before the experiment, each group of rats in vivo insulin weresignificantly increased, the levels of insulin in rats, T3group was the most obvious. Thediabetic group participated in the movement and did not participate in the movement of thediabetic group (V group) compared to the former insulin level significantly increased (P <0.05significant level)(3) a moderate intensity exercise can increase the diabetic exercise group (T1group, T2group,T3group, T4group) content of rat femoral head four intramuscular GLUT4. Among them, themost obvious change in T3group. |
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