A Prelominary Exploration Of INKT Cells In Children With Secretor Otitis Media

Objective To establish whether iNKT cells plays a significant role in Theetiopathogenesis of Secretory Otitis Media (SOM) in children and to find ifThere is any association between iNKT cells and SOM.Methods Peripheral blood samples were collected from10normai children,13children with chronic tonsillitis and adenoids and13children undergoing suigery forSOM. Tonsil samples were collecte from13children with chronic tonsillitis but nigoSOM and7children with chronic tonsillitis and SOM. Adenoid samples were collectefrom13patients with adenoids but no SOM and12patients with adenoids and SOM.Using FCM to detect iNKT cells in all the samplles.Results In the peripheral blood samples of normal children, the frequency of theiNKT cells was （0.9660±0.4221)%. In the peripheral blood samples of chronictonsillitis and adenoids children, the iNKT cells was (1.5231±1.0226)%; In theperipheral blood samples of SOM, the frequency of the iNKT cells wa （1.5692±0.7200）%. The difference was significant(P＜0.05). Inthe Tonsil samplesof chronic tonsillitis, the frequency of the iNKT cells was (0.8883±0.6419)%. In theTonsil samples of chronic tonsillitis and SOM, the frequency of the iNKT cells was（1.0105±0.5241）%. The difference betweenthe two groups was insignificant(P＞0.05).In the Adenoid samples of adenoids, the frequency of the iNKT cells was（0.8246±0.4289）%. Inthe Adenoid samples of adenoids and SOM, the frequencyofthe iNKT cells was （1.2658±0.8250）%. The difference between the two groups wasinsignificant(P＞0.05).Conclusions SOM appears to be multifactorial disease. iNKT cells may be one ofthe contributing factors in the etiopathogenisis of SOM.