The Detection Of Periodontal Suspicious Pathogens And AST And IL-6in Gingival Crevicular Fluid Of Type â…¡ Pre-diabetes Children

Objective:In this study, PCR method was used to detect five kinds of suspicious pathogen in the subgingival plaque and their relative content of children with type â…¡ pre-diabetes of gingivitis. ELISA and automatic biochemical analysis instrument were used to measure the expression level of IL-6and AST in gingival crevicular fluid, in order to analyse the correlation between the periodontal indicators and gingival flora, IL-6and AST of children with type â…¡ pre-diabetes. Then we can investigate the relationship between the subgingival suspicious pathogenic bacteria, the level of IL-6and AST and gingival health status of type â…¡ pre-diabetes.Methods:In this study, we choose children of6to14years as an object of the study, and we select90children of normal and pre-diabetic state by the detection of glucose (FPG and OGTT),clinical periodontal index examination (including SBI, PLI and PD) in GCF and subgingival plaque sample collection as well as general health status checks. We divided these children into three groups in accordance with the glucose metabolic status and periodontal status, including pre-diabetes with gingivitis (group A), normal metabolism associated with gingivitis (group B), the normal metabolism and periodontal healthy (group C), and there were30people in each group. The following laboratory tests are as follows:(1) PCR was used to detect the suspicious pathogens in subgingival plaque.(2) An automatic biochemical analyzer (type7070, HITACHI,Japan) was used to detect the level of AST in GCF samples.(3) ELISA was used to measure the concentration of IL-6in GCF samples.Results:1. The PD of group A was significantly higher than groups B and C, and the difference is statistically significant (P<0.05);the PLI,SBI of group A were higher than group B and C, the difference is significant (P<0.05) between group A and C, but without significant difference between group A and B (P>0.05)(Table1).2. The detection rate of5kinds of suspicious pathogen of group A and B are higher than group C, and the difference is significant(P<0.05),but there was no significant difference between group A and B (P>0.05).(Table3)3. The relative content of Pg, Tf, of Aa of group A is higher than groups B and C, there was statistically significant difference between group A and C(P<0.05), but no with group B (P>0.05). The relative content of five kinds of suspicious have positive correlation with PD, SBI (P<0.05). The relative content of Pg, Tf, of Aa have statistically significant positive correlation between the level of FPG (P<0.05).(Table4,5)4. The IL-6concentration of group A was significantly higher than groups B and C, group B is higher than group C, and the difference was statistically significant (p<0.05).The concentration of AST group A is higher than group B and C. There is statistically significant difference between group A and C (P<0.05),but no with group B (p>0.05).Conclusions:1. The type â…¡ pre-diabetic patients suffering from higher level gingival inflammation of than the simple gingivitis, so we should pay more attention to the daily plaque control to prevent periodontitis to occur.2. Compared to the simple gingivitis, the kinds and amount of periodontal pathogens of type â…¡ pre-diabetes has not changed significantly, but this does not exclude the development of Pg, Aa become the dominant bacteria and continue to lead to periodontitis.3. The concentration of IL-6and AST increasing with the glucose concentration, while with the gingival inflammation process, and become aggravated.So the measurement of AST and IL-6pre-diabetic children may become a dynamic index for evaluation of gingivitis and have clinical significance in early diagnosis of periodontitis.