| Objective:To investigate the effects of tooth extraction on lipid metabolism and carotid intima-media lesions in periodontitis rats with or without diabetes.Methods:49 rats were randomly divided into three groups:A?normal control group?,B?chronic periodontitis,CP group?,group C?diabetes mellitus and chronic periodontitis,DM+CP group?,After the lesion model was established,B and C groups were randomly divided into three groups:B1/C1?natural progression group?,B2/C2?tooth extraction group?,B3/C3?tooth extraction+antibiotic group?.Rat blood was collected at one week before tooth extraction?T1?,one week after the first intervention?T2?,and one?T3?,three?T4?,five?T5?weeks after the second intervention.Detection of TC,LDL-C,HDL-C,TG concentrations in groups A and C.Detection of IL-6 concentrations in groups A,B,and C.Separation of carotid bifurcation sites for HE staining after all rats were sacrificed,measure the carotid intima-media thickness in each group.Statistical analysis of test data.Result:1 Blood glucose and lipids:Fasting blood glucose levels in group C were significantly higher than those in group A at each time point,no significant changes before and after intervention.By the end of the experiment,there was a significant improvement in lipid metabolism in group C.TC,LDL-C decreased significantly,HDL-C increased?P<0.05?.TG level fluctuations,no significant improvement.2 IL-6:?1?IL-6 in group C is higher than group B.Except T1 point,C1>B1,C2>B2,C3>B3.?2?B:IL-6 levels continue to increase in group B1.IL-6 level peaked at T2 point in B2,B3.B2>B3>B1?P<0.05?,[B1:?218.28±26.73?pg·L-1;B2:?470.28±25.31?pg·L-1;B3:?367.19±22.44?pg·L-1].Fall from point T3,significantly lower than B1 at T4 and T5.?3?C:IL-6 levels continue to increase in group C1.IL-6 levels of C2 and C3 significantly increased at T2,C2>C3>C1?P<0.059?,[C1:?293.31±17.22?pg·L-1;C2:?713.71±55.24?pg·L-1;C3:?643.31±47.12?pg·L-1];Significant decline in T3,at this point C2 is still the highest,and significantly lower than C1 at T4 and T5.3 HE stainingGroup A has normal blood vessel morphology;The overall cIMT in group B was significantly thicker than that in group A,[A:?93.8793.87±6.33??m;B:?100.66±26.97??m].In the group,B2>B3>B1.B2 disarranged elastic fibers,small plaques in the media.Lesion in group B3 was lighter than group B2.The overall cIMT in group C is thinner than that in group A and B,[C:?82.98±14.64??m],in the group,C2>C3>C1.In the C2 group,the middle elastic fiber breaks,and plaque increases,vascular breakage after partial staining.Conclusions:1 In this experiment,no improvement was found in fasting blood glucose.The lipid metabolism in DM+CP rats was significantly improved,Although influenced by dietary factors,the independent effect of tooth extraction is less pronounced,but it helps to speed up lipid-lowering,has a positive effect on lipid metabolism.2 Diabetes can increase the degree of inflammation in patients with periodontitis,so that the body is in a state of high levels of inflammatory factors.3 Extraction of teeth will cause acute inflammation in the short term and dramatic increase in systemic inflammatory factors.The use of antibiotics can significantly reduce the acute inflammation IL-6 concentration and accelerate the rate of inflammation.4 Atherosclerosis development is closely related to inflammatory factors.The highest level of IL-6 was found after extraction in CP and DM+CP groups,most severe vascular lesions.The use of antibiotics can significantly reduce the development of vascular lesions. |
PDF Full Text Request