The Effect Of Mild Hypothermia On BCL-2、BAX And Caspase-3in Brain Tissue Of Rats After Heart Arrest

Objective:To explore The Effect of mild hypothermia on BCL-2、BAX and Caspase-3in brain tissue of rats after heart arrest.Methods:The models of CPR were induced by asphyxia. Fifteen male SD rats were randomly divided into normal temperature group, mild hypothermia group1and mild hypothermia group2, with5rats in each group. The rats in normal temperature group were treated with common treatment under room temperature(25℃) after the models were established. The rats in the mild hypothermia group1and mild hypothermia group2were treated with mild hypothermia half an hour later after the model was established. The brain tissue of the rats in each group were obtained after treatment for12hrs.The change in expression of Caspase-3in cerebral cortex neurons in rat was determined with immunohistochemistry (IHC), the change in expression of Bcl-2、Bax in cerebral cortex neurons in rat was determined with western-blots and pathologic changes in brain tissue were observed in both groups.Results:Compared with normal temperature resuscitation group, the expressions of Bcl-2of the hypothermia resuscitation groups have obviously increased(P<0.05),especially in hypothermia group2. The expressions of Bax have no difference(P>0.05).And, the expressions of Caspase-3of the hypothermia resuscitation groups have obviously decreased especially in hypothermia group2(P<0.05).Conclusion:1.Cardiac arrest could lead to brain injury by apoptosis.2.Mild hypothermia could relieve brain injury by restraining the apoptosis of neure.3.Mild hypothermia of32℃could contribute more to the protection of brain tissue than mild hypothermia of34℃.